Regulation of LPS-induced elevation of Ca2+ intracellular level of alveolarmacrophages in chronic bronchitis by Angelica Sinensis and nifedipine |
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Authors: | PENG Ze ZHANG Zhen-xiang XU Yong-jian LIU Zhuo-la SONG Man-jing |
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Affiliation: | 1. Division of Pulmonary Medicine, Tongji Hospital of Tongji Medical University, Wuhan 430030, China; 2. Division of Pulmonary Medicine the Second Affiliated Hospital, Shanxi Medical University, Taiyuan 030001, China |
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Abstract: | AIM: To explore regulation of lipopolysaccharide (LPS)-induced elevation of Ca2+ intracellular level in alveolar macrophages(AMs) from patients with chronic bronchitis by Angelica Sinensis and nifedipine.METHODS:AMs was obtained from 7 patients with chronic bronchitis and 6 normal controls by bronchoalveolar lavage and intracellular Calevel was detected after adding Angelica Sinensis, nifedipine or LPS to the supernatant of AMs loaded by Fura-2. RESULTS: In contrast with normal control group (99.65±32.21 nmol/L), intracellular Ca2+ level in AMs from chronic bronchitis group (189.47±23.69 nmol/L) was increased significantly in the absence of extracellular Ca2+ but not 1 mmol/L. Intracellular Ca2+ level in AMs from chronic bronchitis group were significantly increased by adding 10 μg/mL LPS to the supernatant of AMs. LPS-induced elevation of intracellular Ca2+ level in AMs from chronic bronchitis group was completely inhibited by Angelica Sinensis or nifedipine.CONCLUSION: Both Anelica Sinensis and nifedipine may inhibit activation of AMs from patients with chronic bronchitis by reducing LPS-induced elevation of intracellular Ca2+ level in AMs, suggested that these two medicines may inhibit non-specific inflammation of airways in chronic bronchitis. |
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Keywords: | Angelica Sinensis Nifedipine Bronchitis Macrophages Calcium |
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