Injuring effect of DMSO-soluble particles from cigarette smoke on human umbilical vein endothelial cell line EA. hy 926 in vitro |
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Authors: | HU Xiao-lan XU Cang-bao ZHANG Ya-ping ZHANG Xiao QUAN Ping |
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Institution: | Department of Pathophysiology, Xi'an Jiaotong University, Xi'an 710061, China |
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Abstract: | AIM: To investigate the injuring effect of DMSO-soluble particles from cigarette smoke(DSP) on human umbilical vein endothelial cells. METHODS: Human umbilical vein endothelial cell line EA. hy 926 was used as target cells in the study. The growth and viability of the cells treated with various dosages (1, 2, 4 or 4 mL/L) of DSP and low dose (2 mL/L) of DSP at different time points were evaluated by MTT colorimetric assay and celllular protein assay in 96-well plates. Transmission electron microscopy study was carried out to observe the ultrastructure of human umbilical vein endothelial cells under DSP treatment.RESULTS: DSP inhibited the proliferation of human umbilical vein endothelial cell line EA. hy 926. Under DSP treatment, the reducing cellular protein and increasing cell death(mainly necrosis) were observed in time-dependent and dosage-dependent manners.CONCLUSIONS: These results indicated that the toxic effect of DSP caused functional disturbance and structural damage of human endothelial cells. |
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Keywords: | Umbilical veins Endothelium Cell line Smoking Toxicity |
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