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The effect of vasoactive substances on synthesis and release of C-type natriuretic peptide in cultured human endothelial cells
Authors:WANG Xiao-hong  CHEN Ya-hong  QI Yong-fen  LI Shu-lian PANG Yong-zheng  LIU Nai-kui  TANG Chao-shu
Institution:Institute of Cardiovascular Research, the First Hospital, Beijing Medical University, Beijing 100034, China
Abstract:AIM:To investigate the effect of endothelin (ET), angiotensin II (AngII) and homocysteine (Hcy) on C-type natriuretic peptide (CNP) synthesis and release. METHODS: Human endothelial cell was cultured; CNP was measured by radioimmunoassay method. RESULTS: ET and AngII could augment CNP synthesis in human endothelial cells. Compared with control group, 10-9,10-8,10-7 mol/L ET and Ang II increased CNP content of endothelial cells by 1%(P>0.05), 49%(P<0.05),117%(P<0.01) and 137% (P<0.01),165%(P<0.01),201%(P<0.01),respectively. A great dose of ET and Ang II also stimulated CNP release from cultured human endothelial cells. Hcy had no effect on CNP synthesis, but 10-9,10-8,10-7 mol/L Hcy enhanced CNP release from cultured human endothelial cells by 17%(P>0.05),84%(P<0.01) and 555%(P<0.01), respectively. CONCLUSION: ET, AngII and Hcy might be involved in the synthesis and release of human endothelial cell CNP.Fig 1 Time-course of CNP syntheis and release in cultured human endothelial cell ( ±s,n=6)
Keywords:Natriuretic hormone  Endothelins  Angiotensins  Homocysteine  
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