Effect of Aima recipe on the protein and mRNA expression of TNFα and ICAM-1 in the bronchus of rats with chronic bronchitis |
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| Authors: | TANG Guang-hua HUANG Qi-fu JIANG Liang-duo |
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| Institution: | 1. Beijing Dongzhimen Hospital, Beijing University of Traditional Chinese Medicine, Beijing 100700, China;
2. Department of Pathology, Beijing University of Traditional Chinese Medicine, Beijing 100029, China |
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| Abstract: | AIM:To study the effect of Aima recipe (AM), a traditional Chinese medicine, on the protein and mRNA expression of TNFα and ICAM-1 in the bronchus of rats with chronic bronchitis(CB).METHODS:15 male Wistar rats were randomly divided into three groups: control group, chronic bronchitis (CB) group, CB plus AM group. The protein and mRNA expression of TNFα and ICAM-1 were assayed by immunohistochemistry and in situ hybridization methods.RESULTS:TNFα, ICAM-1 protein and mRNA were more strongly expressed in the area of bronchial epithelium in the CB group compared with control group (P＜0.01), treatment with AM significantly decreased their expressions(P＜0.05). CONCLUSION:Downregulating the expressions of TNFα and ICAM-1 mRNA and protein in the area of bronchial epithelium may be one of the mechanisms underlying therapeutic effect of AM on chronic bronchitis. |
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| Keywords: | Lipopolysaccharides Bronchitis Chinese herbs Tumor necrosis factor RNA messenger In situ hybridization Rats |
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