Effect of chronic hypoxia on [Ca2+]iin pulmonary artery endothelial cells and smooth muscle cells under acute hypoxia |
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Authors: | LIU Chen-hong LIU Sheng-yuan WANG Di-xun |
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Affiliation: | Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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Abstract: | AIM AND METHODS: Using Ca2+-sensitive fluorescent probe Fura-2,we measured the changes of [Ca2+]iin cultured rat pulmonary artery endothelial cells (PAEC) and porcine pulmonary artery smooth muscle cells (PASMC) from normoxic (NC group) or chronic hypoxic group (CH group) when they were exposed to acute hypoxia. RESULTS: The increase in [Ca2+]iin 6th passage of PASMC caused by acute hypoxia in CH group was significantly lower than that in the same passage of NC group (P<0.05).On the contrary, in PAEC, the acute hypoxia induced increase in _i, which was significantly higher in 5th passage of CH group than that in NC group (P<0.05). CONCLUSION: The decrease of the elevation of [Ca2+]icaused by acute hypoxia in PASMC of CH group indicated that it functioned to lower the constrictive response to hypoxia.The intensive increase in [Ca2+]icaused by acute hypoxia in PAEC of CH group might lead to more relaxing factors derived from PAEC,which results in a decrease in HPV. |
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Keywords: | Anoxia Muscle smooth Endothelium Cells |
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