The activative effect of Paecilomyces cicadaeon peritoneal and alveolar macrophages of rats

AIM:To explore the activative effect of water extract from cultured mycelium ofPaecilomyces cicadae (P. cicadae)on peritoneal macrophages (PMΦ) and alveolar macrophages (AMΦ) of rats.METHODS:The rats were randomly divided into four groups: normal control group(Ⅰ), cyclophosphamide (Cy) group (Ⅱ),P. cicadaegroup(Ⅲ), Cy+P.cicadaegroup(Ⅳ).The rats were bred in the same circumstance and were administered with corresponding drugs by subcutaneous injection for 26 days. PMΦ and AMΦ of rats were irrigated by normal saline and collected by centrifuge and incubated in a humidified incubator for 2 h at 37 ℃. The activities of acid phosphatase (ACP) and lactate dehydrogenase (LDH) in the PMΦ and AMΦ were determined by biochemical methods, and the capability of PMΦ and AMΦ in phagocytosis of neutral red were measured by colorimetric method too. RESULTS:After administering P. cicadaeto rats, the activities of ACP and LDH in PMΦ and AMΦ of normal rats were elevated significantly,and the reduction of the activities of ACP and LDH in PMΦ and AMΦ of rats due to Cy was notably antagonized, and the capability of PMΦ and AMΦ phagocytizing the neutral red were strengthened significantly.CONCLUSION:P. cicadaecan activate the PMΦ and AMΦ of rats.