|
|||||
|
|
Development of a quantification assay for the cysts of the toxic dinoflagellate <Emphasis Type="Italic">Alexandrium tamarense</Emphasis> using real-time polymerase chain reaction |
|
| Authors: | |
| Institution: | Laboratory of Marine Microbiology, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502,;Department of Ecosystem Studies, School of Environmental Science, The University of Shiga Prefecture, Hikone, Shiga 522-8533, and;National Research Institute of Fisheries and Environment of Inland Sea, Harmful Algal Bloom Division, Toxic Phytoplankton Section, Ohno, Saeki, Hiroshima 739-0452, Japan |
| Abstract: | ABSTRACT: The cysts of toxic dinoflagellate Alexandrium tamarense are the seed population for the bloom responsible for paralytic shellfish poisoning (PSP). However, it is impossible to identify the Alexandrium spp. cyst on the basis of morphological features. In this study, we prepared A. tamarense cysts by sexual conjugation in laboratory conditions and developed an efficient DNA extraction method for polymerase chain reaction (PCR) assay. Using the A. tamarense cysts, we established the identification and quantification method showing the species specificity and the high sensistivity for A. tamarense cysts using real-time PCR. This assay was also able to detect and quantify the A. tamarense cysts accurately when mixed with excess cysts of A. catenella (Whedon and Kofoid) Balech prepared by conjugation experiment. |
| Keywords: | Alexandrium tamarense cyst dinoflagellate paralytic shellfish poisoning (PSP) real-time PCR |
| 本文献已被 SpringerLink 等数据库收录！ | |