耐盐基因Hal1的克隆及表达载体的构建 |
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引用本文: | 沈雁,陈霞,陈燕,龚毅,陈宇光.耐盐基因Hal1的克隆及表达载体的构建[J].现代农业科技,2009(7):243-244. |
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作者姓名: | 沈雁 陈霞 陈燕 龚毅 陈宇光 |
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作者单位: | [1]中科院上海生命科学研究院,上海200233 [2]上海大学生命科学院,上海200233 |
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摘 要: | 以酵母菌株BWG1-7A基因组DNA为模板,利用PCR方法,扩增出耐盐基因Hal1,测序表明该基因全长为885个核苷酸,与已发表的序列NC_001148比较,同源性99.3%。将该基因插入表达载体pYES2的BamHI和EcoRI酶切位点之间。构建表达载体pYES2-Hal1,序列测定完全正确,为植物表达载体的构建打下基础。
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关 键 词: | 耐盐性 Hal1基因 克隆 |
Cloning of Hal1 gene and construction of its expression plasmid |
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Authors: | SHEN Yan CHEN Xia CHEN Yan GONG Yi CHEN Yu-guang |
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Institution: | 1Research Center of Biotechnology Shanghai Institutes for Biological Sciences the chinese Academy of Sciences;Shanghai 200233;2School of life Sciences;Shanghai University |
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Abstract: | Taking Saccharomyces cerevisiae BWG1-7A genomic DNA as template,Hal1 gene is amplified by PCR.The amplified product was digested with BamHI and EcoRI enzymes,then ligated into pYES2 vector.The recombinant pYES-Hal1 was successfully constructed and verified by DNA sequence,which provided a foundation of the construction of plant expression vector. |
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Keywords: | Salt tolerance Hal1 gene Cloning |
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