苹果实生树阶段转变特异蛋白质的SDS-PAGE分析

 利用聚丙烯酰胺凝胶电泳技术（SDS-PAGE）分析了6株苹果实生树（‘红玉’ב金冠’）叶片中蛋白质随节位升高的动态变化。结果表明，随节位升高蛋白质带发生变化，共发现了3条阶段转变特异蛋白质带，分子量分别为55 kD、19 kD和17 kD。其中55 kD蛋白质表现为定性变化，有5株实生树在36～75节出现，到101～126节消失；19 kD蛋白质在实生树低节位含量高，在86～100节之后含量突然降低；17 kD蛋白质在实生树低节位含量低，随着植株的发育蛋白质在36～50节含量突然增加并保持稳定，到91～105节后含量突然下降。认为这3种蛋白质是苹果实生树阶段转变的特异蛋白质。

SDS-PAGE Analysis of Phase Change-related Proteins in Apple (Malus domestica Borhk.)

Dynamic changes of proteins in leaves from different nodes in 6 hybrid seedlings (Jonathan × Golden Delicious) were analyzed with SDS-polyacrylamide gel electrophoresis in apple. Three protein bands were found specific in different phases. The molecular weight of the 3 protein bands were approximately 55 kD, 19 kD and 17 kD. The 55 kD protein presented qualitatively between nodes No.36～75 and No.101～126. The change of 19 kD and 17 kD proteins was found quantitatively. In leaf samples taken below nodes No. 86～100, the content of 19 kD protein was higher, but a sharp decrease occurred in leaves above nodes No.100. The content of 17 kD protein kept high between nodes No. 36～50 and No. 91～105. It is therefore suggested that the 3 proteins were phase change-related in apple seedlings.