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甘蓝型油菜附加系与芸薹属A基因组杂交F1的获得与鉴定
引用本文:李利军,丁云花,李成琼,简元才,李丽,丁云花.甘蓝型油菜附加系与芸薹属A基因组杂交F1的获得与鉴定[J].中国农学通报,2009,25(13):23-27.
作者姓名:李利军  丁云花  李成琼  简元才  李丽  丁云花
作者单位:1. 北京蔬菜研究中心,北京,100097;西南大学园艺园林学院,重庆,400715
2. 北京蔬菜研究中心,北京,100097
3. 西南大学园艺园林学院,重庆,400715
摘    要:以甘蓝型油菜-萝卜d染色体附加系do和菜心为材料,通过有性杂交途径获得了F1杂种,并对F1杂种进行了形态学、细胞学和分子标记鉴定。结果表明:附加系do与菜心A3间的正反交均可得到种子;除反交A3×do获得的少部分种子为假杂种外均为真杂种;真杂种的形态特征偏向于附加系do,细胞DNA相对含量介于其亲本之间。

关 键 词:江西省    江西省    生态农业    定量评价
收稿时间:2009-04-16
修稿时间:2009-05-05

Production and Identification of Primary Interspecific Generation Between Rape-Radish with Chorosome-D Addition Lines and Brassica Genome A
Li Lijun,Ding Yunhua,Li Chengqiong,Jian Yuancai,Li Li,Kang Jungen.Production and Identification of Primary Interspecific Generation Between Rape-Radish with Chorosome-D Addition Lines and Brassica Genome A[J].Chinese Agricultural Science Bulletin,2009,25(13):23-27.
Authors:Li Lijun  Ding Yunhua  Li Chengqiong  Jian Yuancai  Li Li  Kang Jungen
Institution:Li Lijun, Ding Yunhua, Li Chengqiong, Jian Yuancai, Li Li,Kang Jungen (1Beijing Vegetable Research Center, Beijing 100097; 2School of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715)
Abstract:F1 hybrids between rape-radish with chorosome-D addition line do and flowering chinese cabbage were aquired through sexual hybridization, and identified by morphological investigation, cytological examination and molecular marker technique. The result indicated that hybrids could be obtained from reciprocal crosses, most were all true hybrids except few seeds from reciprocal cross A3×do, and morphological characters of true hybrids were lean to the addition line do and their relative DNA contents tested by Flow Cytometry lied between their parents.
Keywords:rape-radish addition linezz  Brassicazz  chinese cabbage typezz  hybrid identificationzz  molecular markerzz
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