Flow cytometric analysis of canine umbilical cord blood lymphocytes |
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| Authors: | Otani Isao Ohta Kohei Ishikawa Akira Yamada Takeki Ishinazaka Tsuyoshi Ohtaki Tadatoshi Tsumagari Shigehisa Kanayama Kiichi |
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| Institution: | Laboratory of Veterinary Physiology, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan. otani@brs.nihon-u.ac.jp |
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| Abstract: | Lymphocyte subsets in canine umbilical cord blood were flow cytometrically analyzed and compared with those of the dams' peripheral blood. The proportion of CD3+ T lymphocytes, CD21+CD3- B lymphocytes, and CD3-CD21- non-T non-B lymphocytes in umbilical cord blood was 52.9%, 30.4%, and 16.7%, respectively. T lymphocyte/B lymphocyte ratio was significantly lower in the umbilical cord blood than in the dams' peripheral blood (2.1 +/- 1.4 versus 11.0 +/- 8.1, P < 0.001). In contrast, CD4+ lymphocyte/CD8+ lymphocyte ratio was significantly higher in the umbilical cord blood than in the dams' peripheral blood (7.6 +/- 2.2 versus 1.8 +/- 0.6, P<0.001). These findings clarified the phenotypic characters of canine umbilical cord blood lymphocytes. |
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