鸡减蛋综合征病毒EDSV-HX株的分离鉴定与免疫原性评价

为研究某发病鸡群病料中所分离病毒的遗传特性、致病性及免疫原性,通过流行病学、基因序列测定、血凝和血凝抑制试验,确定所分离的病毒为鸡减蛋综合征病毒(EDSV),并命名为EDSV-HX株。随后制备该毒株的油乳剂灭活疫苗,分别以不同剂量免疫蛋鸡,并根据HI抗体效价将免疫蛋鸡重新分为不同的抗体水平组进行攻毒试验。结果显示:当免疫鸡HI抗体效价不低于7log2时,可为免疫蛋鸡提供有效攻毒保护,且产蛋不受影响。研究表明EDSV-HX株具有高致病性和良好的免疫原性,可作为效力检验用毒及灭活疫苗的候选株。

Isolation,Identification and Immunogenicity Assessment of Egg Drop Syndrome Virus HX Strain

To study the genetic characteristics,pathogenicity and immunogenicity of the virus isolated from suspected samples in the layer farm,the isolated virus was identified as egg drop syndrome virus(EDSV)by the epidemiology,gene sequencing,hemagglutination and hemagglutination inhibition(HI)tests,and then was named as EDSV-HX strain.The oil-adjuvant inactivated vaccine against EDSV-HX strain were prepared and administrated to laying hens with different doses.The immunized laying hens were divided into different groups according to HI antibody titer and then were challenged with EDSV-HX strain.The results showed that this inactivated vaccine was able to provide complete protection against EDSV infection when the EDSV HI antibody titer in immunized chickens was not less than 1∶128(7 log2).This study indicated that EDSV-HX strain had high pathogenicity and good immunogenicity,could be used to evaluate the protective effect of vaccine and as a candidate strain of inactivated vaccine.