Expression of CD4 T cell cytokine genes in the colorectal mucosa of inflammatory colorectal polyps in miniature dachshunds |
| |
Authors: | Hiroshi Ohta Kanae Takada Shidow Torisu Masashi Yuki Yu Tamura Nozomu Yokoyama Tatsuyuki Osuga Sue Yee Lim Masahiro Murakami Noboru Sasaki Kensuke Nakamura Masahiro Yamasaki Mitsuyoshi Takiguchi |
| |
Institution: | 1. Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan;2. Veterinary Teaching Hospital, Department of Veterinary Medicine, Faculty of Agriculture, Miyazaki University, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan;3. Yuki Animal Hospital, 2-99 Kibacho, Minatoku, Nagoya, Aichi 455-0021, Japan;4. Laboratory of Veterinary Teaching Hospital, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan |
| |
Abstract: | Inflammatory colorectal polyps (ICRPs) in miniature dachshunds are recently recognized as a major cause of large bowel diarrhea in this dog breed in Japan. ICRPs are characterized by the formation of multiple small polyps and a space-occupying large polyp in the colorectal area, and are thought to be a novel form of inflammatory bowel disease (IBD). In humans, specific cytokine patterns attributed to T helper (Th)1, Th17 and regulatory T cells have important roles in the pathogenesis of IBD. Thus, the aim of the present study was to assess the gene expression of cytokines of T cell subsets in the colorectal mucosa from dogs with ICRPs. Colorectal mucosal specimens from 10 dogs with ICRPs and 14 control dogs were used in this study. Interferon (IFN)-γ, interleukin (IL)-4, IL-17A and IL-10 mRNA expression was assessed using quantitative real-time PCR. IL-17A mRNA expression was significantly increased in large polyps compared to small polyps and controls. IFN-γ and IL-10 mRNA expression in large polyps were significantly higher than in controls. There was no significant difference in IL-4 mRNA expression among the three groups. IL-17A is thought to play important roles in the pathogenesis of ICRPs. IL-10 up-regulation could oppose the proinflammatory function of IL-17A. |
| |
Keywords: | Cytokine Inflammatory colorectal polyps Miniature dachshund T helper cell |
本文献已被 ScienceDirect 等数据库收录! |
|