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| 一株高抗镉细菌KCd1的分离鉴定及其抗性基因初步研究 | |
| 引用本文： | 曾艳,孙建光,王敏,陈强,高俊莲.一株高抗镉细菌KCd1的分离鉴定及其抗性基因初步研究[J].四川农业大学学报,2010,28(1):46-51. |
| 作者姓名： | 曾艳 孙建光 王敏 陈强 高俊莲 |
| 作者单位： | 1. 北京市农林科学院,农业生物技术研究中心,北京,10009;四川农业大学,资源环境学院,四川,雅安,625014 2. 中国农业科学院,农业资源与农业区划研究所,农业部作物营养与施肥重点实验室,北京,100081 3. 北京市农林科学院,农业生物技术研究中心,北京,10009 4. 四川农业大学,资源环境学院,四川,雅安,625014 |
| 基金项目： | 北京市自然科学基金，国家"863计划" |
| 摘 要： | 从北京凉水河床底泥中分离得到一株能在含450 mg/L CdCl2的LB平板正常生长的高抗镉细菌,编号为KCd1。16S rDNA序列分析结果显示,该菌株与Bacillus cereusATCC 14579T的序列同源性达99%;结合脂肪酸测定与生理生化测定结果,确定该菌株在分类上属于Bacillus cereus。根据GenBank中已发表的抗镉细菌cadA序列设计了巢式引物,以菌株KCd1基因组DNA为模板,扩增得到约500 bp的PCR产物,将该PCR产物克隆于pGEM T-Easy载体,测序结果显示其大小为468 bp,与已报道的Staphylococcus haemolyticus的cadA基因对应序列相似性达到99%,由此可知本实验所获得到468 bp基因片段为cadA基因的一段保守序列。该菌株cadA全长基因的获取工作还有待于进一步展开。 |
| 关 键 词： | 抗镉细菌 16S rDNA cadA基因 序列分析 |
Isolation and Identification of a Bacterial Strain KCd1 with High Resistance to Cadmium and Preliminary Study on its cadA Gene |
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| ZENG Yan,SUN Jian-guang,WANG Min,CHEN Qiang,GAO Jun-lian.Isolation and Identification of a Bacterial Strain KCd1 with High Resistance to Cadmium and Preliminary Study on its cadA Gene[J].Journal of Sichuan Agricultural University,2010,28(1):46-51. | |
| Authors: | ZENG Yan SUN Jian-guang WANG Min CHEN Qiang GAO Jun-lian |
| Institution: | ZENG Yan1,2,SUN Jian-guang3,WANG Min1,CHEN Qiang2,GAO Jun-lian1,2(1.Agro-biotechnology Research Center,Beijing Academy of Agriculture , Forestry,Beijing 100097,2.College of Resource , Environment,Sichuan Agricultural University,Yaan 625014,Sichuan,China,3.Institute of Agricultural Resources , Regional Planning,Chinese Academy of Agricultural Sciences /Key Laboratory of Crop Nutrition , Fertilization of Ministry of Agriculture,Beijing 100081) |
| Abstract: | A bacterial strain with high resistance to cadmium was isolated from the river sediment of Liangshui River in Beijing,and named KCd1.The strain can grow on LB medium containing 450 mg/L CdCl2.16S rDNA sequence analysis demonstrated that strain KCd1 shared 99% sequence identity with the strain ATCC 14579T of Bacillus cereus.The morphological characteristics and the results of physiological and biochemical properties determination and fatty acid analysis of the strain KCd1 were in agreement with those of Baci... |
| Keywords: | 16S rDNA bacteria with resistance to cadmium 16S rDNA cadA gene sequence analysis |
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