基于表型和SRAP 标记的切花菊品种遗传多样性分析

 利用45 个表型性状和SRAP 标记分析56 个切花菊品种的遗传多样性。表型变异分析结果表明：21 个性状表现出品种内一致性高及品种间特异性强；主成分分析发现，主成分贡献值较大的性状有花序直径、花序类型和瓣型等花部性状，其次是叶部和茎杆性状，说明所选用的切花菊品种在分类时应以花部性状为主，叶部和茎杆性状为辅；表型性状基于遗传距离UPGMA 聚类，将56 个切花菊品种分为平瓣类、匙瓣类、桂瓣类、匙瓣-平瓣类和管瓣类，聚类结果大致按照花径-瓣型-花型分类。14 对SRAP 引物组合扩增56 个切花菊品种的DNA，共扩增出454 条带，其中多态性带423 条，占扩增带数的93.17%，多态性含量PIC 值在0.72 ~ 0.89 之间，平均为0.82，说明切花菊品种在分子水平上具有丰富的遗传多样性。基于SRAP 标记的UPGMA 聚类分析显示：品种间遗传相似系数在0.64 ~ 0.97 之间，将56个切花菊品种分为平瓣类-匙瓣、桂瓣类和管瓣类，聚类结果大致按照花径-瓣型-花型分类。Mantel检验相关性系数为0.682，两种聚类结果有相似之处，均能很好体现试验切花菊品种间的遗传关系。

Genetic Diversity Analysis in Cut Chrysanthemum Cultivars Based on Morphology and SRAP Markers

Based on phenotypic analysis and SRAP markers，genetic diversity among 56 cultivars ofcut chrysanthemum were analyzed. Variation analysis showed that there were 21 characters whichpresented the high consistency within cultivars and the distinct specificity between cultivars. Principalcomponent analysis showed that flower characters such as inflorescence diameter，inflorescence form andpetal type et al contributed mainly to the principal components，while leaf and stem characters as a secondary contribution. The results indicated that it would be necessary to primarily consider the flower traits，thenleaf and stem traits，as the criterion of classification and identification for cut chrysanthemum. PhenotypicUPGMA clustering analysis showed that 56 cut chrysanthemum cultivars were classified into flat type，spatulate type，irregular type，spatulate-flat type and tubiform type groups，which were mainly accordingto three-grades classification as Inflorescence diameter–Petal type–Inflorescence form. 14 SRAP primerpairs were screened and generated 423 polymorphic bands among 454 bands in total，which the percentageof polymorphic bands reached 93.17%. The values of polymorphism information content（PIC）rangedfrom 0.72 to 0.89 with an average of 0.82. These results indicated there was abundant genetic diversityamong cut chrysanthemum cultivars. UPGMA clustering analysis with SRAP markers showed 56 cultivarswith the genetic coefficient ranged from 0.64 to 0.97 were divided into spatulate-flat type，irregular typeand tubiform type groups，which were also according to three-grades classification as Inflorescencediameter–Petal type–Inflorescence form. Mantel’s test with correlation coefficient of 0.682 indicated thatthe high accordance between phenotypic and molecular markers analysis，suggesting both analysis couldwell reflect the genetic relationship of cut chrysanthemum cultivars.