激素和非生物胁迫对月季RhPIP1;1 启动子活性的调节作用

 通过反向PCR 方法克隆得到月季（Rosa hybrida L.）‘萨蔓莎’质膜型水孔蛋白基因RhPIP1;1 上游2 126 bp 的启动子序列。PLACE 分析发现，RhPIP1;1 启动子序列中含有GA、ABA 和乙烯等激素诱 导相关顺式作用元件及干旱、低温和盐胁迫等非生物胁迫相关顺式作用元件。在RhPIP1;1promoter：：GUS 转基因拟南芥中发现，RhPIP1;1 启动子活性与植株发育进程相关；几乎所有器官均可检测到GUS 表达， 而且在迅速扩展的器官以及叶片和花的维管组织中尤为强烈。同时在6 d 和9 d 苗龄的转基因植株中，GA 处理上调了莲座叶中RhPIP1;1 启动子的活性，而ABA、甘露醇、NaCl 和冷处理分别下调了莲座叶和根 中RhPIP1;1 启动子的活性。将RhPIP1;1 启动子不同长度的5′端缺失片段融合GUS 基因，并在烟草叶片 中瞬时表达，缺失–580 ~–256 之间的324 bp 片段后，启动子活性显著降低。研究结果表明RhPIP1;1 启 动子对多种激素和非生物胁迫存在响应，并且这种响应具有发育和器官特异性；RhPIP1;1 启动子中–580 ~ –256 区段对于启动子活性具有重要的作用。

Regulation of the Rose RhPIP1;1 Promoter Activity by Hormones and Abiotic Stresses

A 2 126 bp sequence of the RhPIP1;1 promoter was isolated from rose（Rosa hybrida L. ‘Samantha’）through inverse PCR. PLACE analysis indicated that the RhPIP1;1 promoter harbors hormone-responsive elements，such as GA，ABA，and ethylene related cis-elements，and abiotic stresses-responsive elements，such as dehydration-，salt-，and cold-related cis-elements. In RhPIP1;1promoter：：GUS-expressing Arabidopsis，the activity of RhPIP1;1 promoter was found to be associated with the development process. Almost all the organs exhibited the promoter activity，which is particularly active in expanding organs and vascular tissues of leaves and flowers. Moreover，in the 6- or 9-day-old transgenic plants，the promoter activity was up-regulated by GA treatment in the rosettes， whereas down-regulated by ABA，mannitol，NaCl，and cold treatment in the rosettes and roots. A series of 5′ deletion fragments of the RhPIP1;1 promoter were fused with GUS gene，and transiently expressed in tobacco leaves. It was found that deleting 324 bp fragment from–580 to–256 led to a significant decrease of the promoter activity. Taken together，our results demonstrated that the RhPIP1;1 promoter responds to various hormones and abiotic stresses in a developmental- and spatial-dependent manner. And the–580 to–256 region is important for the activity of the RhPIP1;1 promoter.