| 鸡传染性支气管炎病毒在鸡胚肾细胞和气管环培养中的适应性比较 |
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| 引用本文： | 吴延功,郑明球,吴增坚,蔡宝祥,陈溥言.鸡传染性支气管炎病毒在鸡胚肾细胞和气管环培养中的适应性比较[J].南京农业大学学报,1995(2). |
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| 作者姓名： | 吴延功 郑明球 吴增坚 蔡宝祥 陈溥言 |
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| 作者单位： | 南京农业大学动物医学院 |
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| 摘 要： | 将６株鸡传染性支气管炎病毒（ＩＢＶ）分别接种鸡胚肾细胞（ＣＥＫＣ）和气管环培养（ＴＯＣ）。６株病毒无论是否已经适应于鸡胚，都在气管环培养中能引起病变。病毒适应于ＣＥＫＣ，是与病毒在鸡胚和细胞培养上的传代次数有关。分别用ＳＰＦ鸡胚、非免疫鸡胚和普通鸡胚制备的ＴＯＣ测定ＩＢＶ－Ｍ４１株的ＩＤ５０，结果用ＳＰＦ鸡胚和非免疫鸡胚制备的ＴＯＣ测定的ＩＤ５０都获得较高滴度，而在普通鸡胚制备的ＴＯＣ中ＩＤ５０明显低。
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| 关 键 词： | 鸡传染性支气管炎病毒 鸡胚肾细胞 气管环培养 细胞病变 |
COMPARATIVE SENSITIVITIES OF CHICKEN EMBRYO KIDNEY CELL CULTURES AND TRACHEAL ORGAN CULTURES TO INFECTIOUS BRONCHITIS VIRUS |
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| Wu Yangong, Zheng Mingqiu, Wu Zengjian, Cat Baoxiang and Chen Puyan.COMPARATIVE SENSITIVITIES OF CHICKEN EMBRYO KIDNEY CELL CULTURES AND TRACHEAL ORGAN CULTURES TO INFECTIOUS BRONCHITIS VIRUS[J].Journal of Nanjing Agricultural University,1995(2). |
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| Authors: | Wu Yangong Zheng Mingqiu Wu Zengjian Cat Baoxiang and Chen Puyan |
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| Abstract: | Studies in vitro with chicken embryo kidney cell (CEKC) cultures and tracheal orgsn cultures (TOC)were attempted to assess the pathogenicity of six strains of infectious bronchitis viruses (IBV) (M41, Connecticut, T,Gray, GIBV and NIBV). The pathogenicity in TOC was found whether these viruses had been adapted to embryo ornot. However, the adaption of viruses on CEKC was related with the passage times in embryo or on cell culture. InTOC prepared from SPF and un-immunized chicken embryos the infective titers of M41 strain were higher than thatfrom immunized chicken embryos |
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| Keywords: | avian infectious bronchitis virus chicken embryo kidney cell tracheal organ culture cytopethic effect |
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