A C-type lectin with antibacterial activity in weather loach,Misgurnus anguillicaudatus |
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Authors: | Xiao-Wen Zhang Cong-Hui Yang Hong-Quan Zhang Xin-Tong Pan Ze-Yu Jin Hong-Wei Zhang Xiao-Hua Xia |
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Affiliation: | 1. College of Life Science, Henan Normal University, Xinxiang, China;2. Qingdao West Coast New Area No.1 High School, Qingdao, China;3. Department of Nature Resources, Henan Institute of Science and Technology, Xinxiang, China |
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Abstract: | C-type lectins are carbohydrate-binding proteins that play important roles in immunity by serving as pattern recognition receptors. In the present study, a novel nattectin-like C-type lectin was obtained from the weather loach, Misgurnus anguillicaudatus, designated as MaCTL. MaCTL encodes a peptide with 165 amino acids, with a signal peptide and a single C-type lectin domain (CTLD), containing a galactose-specific QPD motif and a conserved Ca2+-binding site. Transcripts of MaCTL were significantly upregulated after immune challenge with its pathogen A. hydrophila. In vitro assays with recombinant MaCTL protein revealed that it exhibited hemagglutinating and bacterial agglutinating activities, in a Ca2+-dependent manner. MaCTL was found to bind to a wide range of bacteria, as well as bind to bacterial polysaccharides LPS and PGN. Moreover, MaCTL displayed antimicrobial activity by inhibiting the growth of bacteria. These results collectively suggest that MaCTL is involved in the antibacterial defence of weather loach. |
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Keywords: | agglutination antimicrobial C-type lectin innate immunity loach pattern recognition receptor |
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