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| MFLP分子标记技术在花生上的应用初探 | |
| 引用本文： | 李杨,韩柱强,熊发前,王育荣,高国庆. MFLP分子标记技术在花生上的应用初探[J]. 花生学报, 2010, 39(3): 26-29 |
| 作者姓名： | 李杨 韩柱强 熊发前 王育荣 高国庆 |
| 作者单位： | 1. 广西大学农学院,广西,南宁,530005 2. 广西农业科学院经济作物研究所,广西,南宁,530007 3. 广西作物遗传改良生物技术实验室,广西,南宁,530007 |
| 基金项目： | 广西农科院科技发展基金资助 |
| 摘 要： | 采用MFLP标记技术对两个栽培种花生DNA进行扩增分析。从160对MFLP引物中筛选出41对多态性条带引物,引物多态率为25.63%;每对多态性引物扩增带数约23～56条（其中多态性条带1～3条）,共检测到差异条带59条;其中约有29条表现显性多态,15对表现共显性多态,共显性标记出现频率为34.09%。研究结果表明,MFLP标记在栽培种花生中具有较丰富的多态性和较高的共显性率,在遗传多样性分析和分子标记辅助育种等方面具有良好的应用前景。 |
| 关 键 词： | 花生 MFLP 分子标记 |
Preliminary Study of MFLP in Peanut (Arachis hypogaea L.) |
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| LI Yang,HAN Zhu-qiang,XIONG Fa-qian,WANG Yu-rong,GAO Guo-qing. Preliminary Study of MFLP in Peanut (Arachis hypogaea L.)[J]. Journal of Peanut Science, 2010, 39(3): 26-29 | |
| Authors: | LI Yang HAN Zhu-qiang XIONG Fa-qian WANG Yu-rong GAO Guo-qing |
| Affiliation: | 1.Agricultural College,Guangxi University,Nanning 530005,China;2.Cash Crops Research Institute,Guangxi Academy of Agricultural Science,Nanning 530007,China;3.Guangxi Crop Genetic Improvement and Biotechnology Lab,Nanning 530007,China) |
| Abstract: | 160 MFLP primer pairs were employed to study polymorphism within two peanut parent genotypes,41 of which produced 1～3 differentiate bands in total 23 to 56 bands.The percentage of polymorphic primer pairs was 25.63% and total 59 polymorphic bands were detected.29 of 59 polymorphic bands showed dominant,and 15 showed co-dominant with percentage of 34.09%.These results demonstrated that MFLP has high polymorphism and co-dominant rate in peanut,which will be helpful to genetic diversity analysis and molecular marker-assisted breeding. |
| Keywords: | MFLP |
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