猪屎豆与淡紫拟青霉联合防治烟草根结线虫病的效果评价

为明确猪屎豆与淡紫拟青霉对烟草根结线虫病的联合作用，以猪屎豆根系粉为材料，研究了猪屎豆根系水提液对淡紫拟青霉的生长、产孢量、孢子萌发率及南方根结线虫致死率的影响，同时设置3个处理（单独间作猪屎豆、单独施用淡紫拟青霉、间作猪屎豆同时施用淡紫拟青霉）对猪屎豆与淡紫拟青霉联合防治烟草根结线虫病的协同增效效应进行了综合评价。结果表明：（1）猪屎豆根系粉适能促进紫拟青霉的生长、产孢及萌发，0.01 g/mL猪屎豆根系粉的淡紫拟青霉培养物的水提液处理南方根结线虫J2，24 h校正致死率高达96.08%。（2）烤烟间作猪屎豆同时施用淡紫拟青霉，能显著增加烤烟的株高和叶片宽度，烟草根结线虫病的防效高达67.5%，较单独间作猪屎豆的防效提高了22.9%，较单独使用淡紫拟青霉的防效提高了10.2%。本研究认为猪屎豆与淡紫拟青霉协同作用防治烟草根结线虫具有显著的协同增效效应。

Integrated Control of Tobacco Root-knot Nematode Disease by Crotalaria pallida and Paecilomyces lilacinus

The aim is to identify the integrated effect of Crotalaria pallida and Paecilomyces lilacinus against tobacco root- knot nematode disease. The root powders of C. pallida were used as materials to study the influences on the growth, sporulation quantity, spore- germination rate of P. lilacinus, and Meloidogyne incognita J2 corrected death. While three treatments (single intercropping C. pallida, single using P. lilacinus, intercropping C. pallida at the same time using P. lilacinus) were set to make a comprehensive evaluation on integrated C. pallida and P. lilacinus against tobacco root- knot nematode disease. The results showed as follows: (1) the root powders of C. pallida promoted the growth, sporulation quantity, and spore-germination rate of P. lilacinus, under the treatment of 24 h and 0.01 g/mL root powders water extracts of C. pallida and P.lilacinus spore for Meloidogyne incognita J2, the corrected death rate was 96.08%; (2) tobacco intercropping with C. pallida and using P. lilacinus simultaneously could significantly increase the plant height and maximum width of tobacco leaves, the control efficiency was 67.5%, increased by 22.9% and 10.2% compared with single intercropping C. pallida and single using P. lilacinus, respectively. Taken together, C. pallida and P. lilacinus have obvious synergistic effect on controlling tobacco root-knot nematode disease.