EST-SSR和RAPD标记检测油菜(Brassica napus)遗传多样性

亲本选配是杂种优势利用中强优势组合配制的关键环节,遗传距离决定优势强弱。本文采用EST-SSR和RAPD两种分子标记技术检测甘蓝型油菜4份不育系、3份保持系1、0份恢复系和11份常规品种共35个材料的遗传多样性及与杂种优势的关系。结果共检测到399个等位位点,其中130个等位变异,占总检测位点的32.57%。NTSYS软件聚类分析结果表明:35份材料遗传距离范围为0.0280~0.2801,在遗传距离(GD)0.1401下分为6类,3个不育系Ogu、Polima和陕2A聚在第V类,Xin1 CMS(新1号不育系)遗传距离较远;不育系之间的遗传差异比不育系与保持系、恢复系及常规品种之间小,基本反映了品种(系)的遗传基础和系谱来源。

DIVERSITY ANALYSIS OF Brassica napus BY EST-SSR AND RAPD MARKERS

Parents matching is the key point in the strong portfolio for heterosis application.Heterosis strength is distance decided by genetic distance.35 varieties of Brassica napus,including 4 sterile lines,3 maintainer lines,10 restorer lines and 11 conventional varieties,were analyzed by EST-SSR and RAPD markers for the genetic diversity and heterosis.399 alleles were detected in the 35 varieties,of which 130 alleles accounting for 32.57% showed polymorphic.The range of the 35 varieties'genetic distance was between 0.0280～0.2801 computed by NTSYS(software).At the level of genetic distance 0.1401,the varieties were divided into 6 groups.Ogu,Polima and Shan 2A CMS were clustered into the fifth groups.Xin1 CMS had further genetic distance.Compared to maintainer lines and sterile lines,restorer lines and conventional varieties,showed more genetic diversity.It was described their diversity of genetic basis and pedigree sources on the whole.