Molecular characterization of genetic diversity in European germplasm of perennial ryegrass |
| |
Authors: | S Bolaric S Barth A E Melchinger U K Posselt |
| |
Institution: | (1) State Plant Breeding Institute, University of Hohenheim, D-70593 Stuttgart, Germany;(2) Teagasc, Crops Research Centre, Oak Park, Carlow, Ireland;(3) Institute of Plant Breeding, Population Genetics and Seed Science, University of Hohenheim, D-70593 Stuttgart, Germany |
| |
Abstract: | Summary Perennial ryegrass (Lolium perenne L.) is the most important grass species for temperate grassland agriculture. The level and distribution of genetic variation
in gene bank ecotype collections is still largely unknown but of great interest for the planning of breeding programs. The
objectives of this study were to (i) assess the molecular diversity of Polish ecotypes of perennial ryegrass, and (ii) compare
the relationship between this group and German ecotypes and European cultivars investigated previously. A total number of
166 polymorphic marker bands were detected among the 171 individual plants of the 9 Polish ecotypes. In a joint analysis with
9 Polish and 22 German ecotypes, and 22 European cultivars 172 polymorphic RAPD markers could be found. Genetic distance among
the Polish ecotypes ranged from 0.31 to 0.51, while for all 53 populations a broader range was detected (0.25–0.67). An analysis
of molecular variance (AMOVA) revealed a much larger variation within populations (71%) than among them (29%). The Polish
ecotypes contained the highest within population variation (74%). The largest among group difference (15%) was found between
the Polish ecotypes versus all other accessions. We conclude that the Polish ecotypes represent a valuable genetic resource
for enlarging the genetic variation in the West European germplasm pool of perennial ryegrass. |
| |
Keywords: | AMOVA diversity ecotypes genetic distance Lolium perenne L RAPD marker |
本文献已被 SpringerLink 等数据库收录! |
|