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| 枯草芽孢杆菌木聚糖酶Xyn B在毕赤酵母中的表达 | |
| 引用本文： | 莫毅,梁方方,赖志强,卢玉发,廖玉英,何仁春,黄丽霞,杨琳.枯草芽孢杆菌木聚糖酶Xyn B在毕赤酵母中的表达[J].中国饲料,2012(9):19-21. |
| 作者姓名： | 莫毅 梁方方 赖志强 卢玉发 廖玉英 何仁春 黄丽霞 杨琳 |
| 作者单位： | 1. 广西人口和计划生育研究中心 2. 广西畜牧研究所 3. 华南农业大学动物科学学院 |
| 摘 要： | 编码枯草芽孢杆菌(Bacillus subtilis strain GD3b)木聚糖酶(Xylanse B,Xyn B)基因从重组载体pUC-XynB中切割,并克隆到pPICZa A载体中,经酶切和测序鉴定,成功构建了分泌型毕赤酵母表达载体pPICZa-Xyn B。载体经线性化后转入毕赤酵母X-33,并筛选获得可通过甲醇诱导分泌表达Xyn B重组蛋白的毕赤酵母工程菌X-33/Xyn B。 |
| 关 键 词： | 木聚糖酶 毕赤酵母 表达 重组蛋白 |
Expression of Bacillus subtilis strain xylanse B gene in Pichia pastouris |
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| Institution: | Mo Yi,Liang Fangfang,Lai Zhiqiang,et al. |
| Abstract: | The Bacillus subtilis strain xylanse B(Xyn B)gene was digested from the recombinant plasmid pUC-Xyn B and cloned into vector pPICZa A.After restriction enzyme digestion and sequencing identifications,the secreted expression pichia vector pPICZa-Xyn B was constructed successfully.The recombinant vector pPICZa-Xyn B was linearized and transformed into Pichia pastoris X-33 to rebuild the Pichia pastoris recombinant strain X-33/Xyn B,which could be secreted express Xyn B recombinant fusion protein with methanol induction. |
| Keywords: | xylanase Pichia pastoris expression recombinant protein |
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