Insect-resistant mechanism of transgenic triploid of Chinese white poplar |
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Authors: | Shengliang Yuan Baojia Gao Na Zhang |
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Institution: | (1) Postgraduate Department, Agricultural University of Hebei, Baoding, 071000, China;(2) College of Plant Protection, Agricultural University of Hebei, Baoding, 071000, China |
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Abstract: | The activities of antidotal enzymes and digestive enzymes of Clostera anachoreta (Fabricius) instar larvae, feeding on leaves of three kinds of insect-resistant clones of transgenic triploid of Chinese
white poplar, after 4, 12, 24, 48, 72 and 96 h, were investigated. The results showed that, feeding on clone 7, the activity
of esterase, carboxylesterase, and mixed-function oxidases in the midgut of the larvae was very much decreased. Feeding on
clone 10, those results were less than those of clone 7 and there were few changes on the larvae, which fed on clone 26. The
changes of the amylase in the midgut of larvae were the same as those described above. However, the activities of glutathione
S-transferase and proteinase were complex, increased markedly after 24 h feeding on clone 7, and then declined rapidly. The
same changes were taking place on the larvae feeding on clone 10. There were many slight changes in glutathione S-transferase
of the larvae, feeding on clone 26; no changes occurred in the proteinases of the midgut. Thus, the antidotal enzymes and
digestive enzymes in the midgut of the larvae were inhibited. This may be the main mechanism of the transgenic triploid of
Chinese white poplar.
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Translated from Journal of Agricultural University of Hebei, 2005, 28(5) 译自: 河北农业大学学报, 2005, 28(5)] |
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Keywords: | transgenic triploid of Chinese white poplar Clostera anachoreta (Fabricius) antidotal enzymes digestive enzymes |
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