| 水稻BWMK1互作基因的分离 |
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| 引用本文： | 潘素君. 水稻BWMK1互作基因的分离[J]. 湖南农业大学学报(自然科学版), 2011, 37(5): 494-496 |
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| 作者姓名： | 潘素君 |
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| 作者单位： | 湖南农业大学作物基因工程湖南省重点实验室 |
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| 基金项目： | 国家自然科学基金（30470990）、国家教育部博士点基金（20060537004）、湖南农业大学作物种质创新与资源利用重点实验室科学基金开放项目（10KFXM13 |
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| 摘 要： | 为探明BWMK1介导的信号传导途径,采用酵母双杂交系统,构建水稻稻瘟病菌诱导后的cDNA文库,以BWMK1为诱饵,对文库进行筛选,通过验证、测序和基因编码分析,获得了7个BWMK1互作基因BWIP1～BWIP7,分别位于水稻基因组第12、2、3、10、6、4、5染色体上;7个互作基因中,除BWlP2和BWIP7未找到同...
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| 关 键 词： | 水稻 稻瘟病和伤诱导的促分裂素原活化蛋白激酶1 互作基因 酵母双杂交 稻瘟病菌 |
| 收稿时间： | 2011-04-02 |
| 修稿时间： | 2011-04-02 |
Identification of rice genes interacting with BWMK1 |
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| pansujun. Identification of rice genes interacting with BWMK1[J]. Journal of Hunan Agricultural University, 2011, 37(5): 494-496 |
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| Authors: | pansujun |
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| Affiliation: | The Key Lab of Crop Genetic Engineering of Hunan Agricultural University |
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| Abstract: | The rice MAPK gene BWMK1 is transcriptionally activated by fungal pathogen infection and wounding and acts as a positive regulator in plant disease resistance signaling. To further understand the BWMK1-mediated molecular signaling regulating pathways, a yeast two-hybrid system using BWMK1 as a bait was used to screen BWMK1 interacting proteins, and 7 BWMK1 interacting protein encoding genes BWIP1BWIP7 were isolated, BWIP1BWIP7 were located on 12, 2, 3, 10, 6, 4, 5 chromosomes, respectively. Homologous coding proteins were found for each isolated gene except BWIP2 and BWIP7, which indicated that BWIP1, 3, 4, 5 and 6 respectively coded for 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase, myo-inositol phosphate synthease, putative phosphate transporter, WD-40 repeat containing protein and N-acetyl glutamate kinase 2. |
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| Keywords: | Key words: rice blast-and wound-induced MAP kinase 1(BWMK1) interacting gene yeast two-hybrid rice blast |
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