Serodiagnosis of Neospora caninum infection in cattle using a recombinant tNcSRS2 protein-based ELISA |
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| Authors: | Liu Jing Yu Jinshu Wang Ming Liu Qun Zhang Wei Deng Chong Ding Jun |
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| Institution: | Parasitology Laboratory, Department of Veterinary Preventive Medicine, College of Veterinary Medicine, China Agricultural University, Beijing, PR China. |
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| Abstract: | The truncated NcSRS2 gene (tNcSRS2) by removal of the N-terminal hydrophobic sequence was cloned into the pGEX-6p-1 plasmid and subsequently expressed as a glutathione-S-transferase (GST) fusion protein. The purified recombinant tNcSRS2 protein was specific to Neospora caninum and was used in an ELISA for the diagnosis of neosporosis. There was a good agreement between tNctSRS2-based ELISA and three commercially available diagnostic kits (IDEXX ELISA, HIPRA ELISA and VMRD IFAT). Three hundred dairy cattle serum samples from 9 regions were tested by our ELISA. The herd prevalence was 100% and the overall prevalence was 20.3%. There was a statistically significant difference in seroprevalence between regions (P<0.01) and an association between abortion history and N. caninum seropositivity. Our study showed that neosporosis in dairy cattle is widespread in China. |
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