Alleviation of drought stress effects in sunflower seedlings by the exopolysaccharides producing <Emphasis Type="Italic">Pseudomonas putida</Emphasis> strain GAP-P45 |
| |
Authors: | V Sandhya Ali SK Z Minakshi Grover Gopal Reddy B Venkateswarlu |
| |
Institution: | (1) Central Research Institute for Dry land Agriculture, Santoshnagar, Hyderabad, 500059, India;(2) Department of Microbiology, Osmania University, Hyderabad, 500007, India; |
| |
Abstract: | Production of exopolysaccharides (EPS) can be used as a criteria for the isolation of stress tolerant microorganisms. In the
present study, EPS-producing fluorescent pseudomonads were isolated from alfisols, vertisols, inseptisols, oxisols, and aridisols
of different semiarid millet growing regions of India and were screened in vitro for drought tolerance in trypticase soy broth
supplemented with different concentrations of polyethylene glycol (PEG6000). Out of the total 81 isolates, 26 could tolerate
maximum level of stress (−0.73 MPa) and were monitored for the amount of EPS produced under maximum level of water stress.
The strain GAP-P45, isolated from alfisol of sunflower rhizosphere, showed the highest level of EPS production under water
stress conditions, was identified as Pseudomonas putida on the basis of 16S rDNA sequence analysis, and was used as seed treatment to study its effect in alleviating drought stress
effects in sunflower seedlings. Inoculation of Pseudomonas sp. strain GAP-P45 increased the survival, plant biomass, and root adhering soil/root tissue ratio of sunflower seedlings
subjected to drought stress. The inoculated bacteria could efficiently colonize the root adhering soil and rhizoplane and
increase the percentage of stable soil aggregates. Scanning electron microscope studies showed the formation of biofilm of
inoculated bacteria on the root surface and this, along with a better soil structure, might have protected the plants from
the water stress. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|