萘二甲酐减轻咪唑乙烟酸对油菜药害的效果和解毒机理初报

采用模拟咪唑乙烟酸土壤残留，以油菜盆栽试验研究萘二甲酐对咪唑乙烟酸土壤残留的解毒效果和解毒机理。结果表明，咪唑乙烟酸25g ai/hm2用量下对油菜有较重药害，可使油菜苗的株高、鲜重等生长指标和谷胱甘肽（GSH）含量、谷胱甘肽-S-转移酶（GST）活力、氨基酸含量等生理指标明显降低。用种子重量0.5%萘二甲酐（NA）湿润拌种处理油菜种子能减轻咪唑乙烟酸对油菜苗的药害。萘二甲酐+咪唑乙烟酸处理的油菜苗株高和鲜重明显高于咪唑乙烟酸处理。萘二甲酐和咪唑乙烟酸都能导致油菜苗的氨基酸含量发生变化，并对GSH含量和GST活力有不同程度的影响。与咪唑乙烟酸25g ai/hm2处理相比，萘二甲酐加入咪唑乙烟酸后，使16种氨基酸含量升高，只有脯氨酸含量降低，氨基酸总量升高；GSH含量略有增加，但GST活力有显著提高。可以认为提高油菜苗氨基酸含量和GST的活力是萘二甲酐对咪唑乙烟酸解毒的重要途径。

Alexipharmic effect of 1,8-naphthalic anhydride on rape from phytotoxicity of imazethapyr

 Simulated experiment was carried out to investigate the alexipharmic effect and mechanism of 1,8-naphthalic anhydride (NA) on the imazethapyr residues in soil using potted rape as bioassay material. The rape seedling height, fresh weight, activity of glutathione-S-transferase (GST), amount of amino acids, and content of glutathione (GSH) were reduced with 25g ai/hm2 imazethapyr. The damage was alleviated under seed dressing with 0.5% (seed weight) NA. Compared to the treatment of imazethapyr, rape seedling height and fresh weight were increased under NA + imazethapyr. The amount of amino acids, content of GSH, and activity of GSTs were induced by both NA and imazethapyr. Under NA + imazethapyr, 16 amino acids (except proline) were increased, GSH was slightly accumulated, while GST activity was significantly stimulated. Results indicated that imazethapyr detoxification was resulted from high amount of amino acid and more active GSTs.