喹胺醇与鸡血浆蛋白结合的初步研究 |
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引用本文: | 高杰,杨志强,李建喜,王学智,兀征,孟嘉仁,张艳,袁晓科. 喹胺醇与鸡血浆蛋白结合的初步研究[J]. 中兽医医药杂志, 2008, 27(4): 5-7 |
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作者姓名: | 高杰 杨志强 李建喜 王学智 兀征 孟嘉仁 张艳 袁晓科 |
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作者单位: | 1. 甘肃农业大学动物医学院,甘肃兰州,730070;中国农业科学院兰州畜牧与兽药研究所 2. 中国农业科学院兰州畜牧与兽药研究所 3. 甘肃省饲料兽药监察所 |
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基金项目: | 中央级公益性科研院所基本科研业务费专项资金资助项目,甘肃省自然科学基金,兰州畜牧与兽药研究所科研基金 |
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摘 要: | 采用平衡透析法结合反相高效液相色谱法(RP—HPLC)测定鸡血浆中喹胺醇的蛋白结合率。色谱条件为ZORBAX Eclipse XDB C18色谱柱(250mm×4.6mm,5μm),流动相为乙腈:水(40:60,v/v),流速1.0mL/min,检测波长352nm。结果显示,喹胺醇浓度为1.1、5.5、22.0μg/mL缓冲液中鸡血浆的蛋白结合率分别为84.41%、75.30%、66.96%;透析平衡时间约为30h。表明喹胺醇的鸡血浆蛋白结合率较高,药物浓度与血浆蛋白结合率之间呈反比关系。
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关 键 词: | 喹胺醇 血浆 蛋白结合率 鸡 |
Study on the integratation between Kui'anchun and plasma proteins of chicken |
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Affiliation: | GAO Jie, YUAN Xiao - ke (College of Veterinary Medicine, Gansu Agricultural University, Lanzhou Gansu 730070, China) |
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Abstract: | The binding rate of Kui'anchun to plasma protein of chicken was determined by using the equilibrium dialysis and reversed- phase high performance liquid chromatography (RP- HPLC). Conditions of RP- HPLC were ZORBAX Eclipse XDB C18 of chromatographic column ( 250 mm × 4. 6 mm ,5μm) , the mobile phase of aeetonitrile - water (40/60, v/v) , the flow rale of 1.0 ml. · min^ -1 and the UV detecting wavelength of 352 nm. The protein - binding rates were 84.41% ,75.30% and 66.96% al conceutration of 1. 1 μg/mL, 5.5 μg/mL, and 22.0 μg/mL in buffer, respectively. The time of dialysis was about 30 hours. The result showed that the binding rate of Kui'anchun to plasma protein was high in chicken, while the protein - binding rate was decreased with the increasing of drugs concentration. |
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Keywords: | Kui'anchun plasma protein - binding rate chicken |
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