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| 水稻白叶枯病菌内源过氧化氢的产生及定位 | |
| 引用本文： | 李欣,于慧春,庞新跃,李红玉,王金生. 水稻白叶枯病菌内源过氧化氢的产生及定位[J]. 南京农业大学学报, 2009, 32(3) |
| 作者姓名： | 李欣 于慧春 庞新跃 李红玉 王金生 |
| 作者单位： | 1. 河南科技大学食品与生物工程学院,河南,洛阳,471003 2. 河南科技大学医学技术与工程学院,河南,洛阳,471003 3. 兰州大学生命科学学院,甘肃,兰州,730000 4. 南京农业大学植物保护学院/农业部病虫监测与治理重点开放实验室,江苏,南京,210095 |
| 基金项目： | 国家自然科学基金项目 |
| 摘 要： | 采用辣根过氧化物酶-酚红法定量检测病原菌细胞内源过氧化氢的产生,并通过组织化学法观察细胞中过氧化氢积累的亚细胞定位.结果表明:辣根过氧化物酶-酚红法及组织化学染色法均检测到水稻白叶枯病菌内源过氧化氢的产生.透射电子显微镜观察结果和辣根过氧化物酶-酚红法定量检测结果均显示,过氧化氢在水稻白叶枯病菌细胞中普遍存在,不同病原菌内源过氧化氢的水平有极显著差异.病原菌细胞中过氧化氢的积累主要定位在细胞壁. |
| 关 键 词： | 白叶枯病菌 过氧化氢 组织化学 定位 |
Production and localization of endogenous hydrogen peroxide in Xanthomonas oryzae pv.oryzae |
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| LI Xin,YU Hui-chun,PANG Xin-yue,LI Hong-yu,WANG Jin-sheng. Production and localization of endogenous hydrogen peroxide in Xanthomonas oryzae pv.oryzae[J]. Journal of Nanjing Agricultural University, 2009, 32(3) | |
| Authors: | LI Xin YU Hui-chun PANG Xin-yue LI Hong-yu WANG Jin-sheng |
| Affiliation: | 1.College of Food and Bioengineering;2.College of Medical Technology and Engineering;Henan University of Scienceand Technology;Luoyang 471003;China;3.School of Life Sciences;Lanzhou University;Lanzhou 730000;4.College of Plant Protection/Key Laboratory of Monitoring and Management of Plant Diseases and Pests;Ministry of Agriculture;Nanjing Agricultural University;Nanjing 210095;China |
| Abstract: | Production and localization of endogenous hydrogen peroxide(H2O2)were investigated in strains of Xanthomonas oryzae pv.oryzae using horseradish peroxidase(HRP)-red phenol assay or by histochemical analysis under transmission electron microscopy.Experiments identified the endogenous H2O2 production in nearly all strains tested.Levels of endogenous H2O2 production were different among these strains,and the accumulated H2O2 was mainly localized in the cell wall,while the H2O2 accumulation was undetectable in p... |
| Keywords: | Xanthomonas oryzae pv.oryzae hydrogen peroxide histochemistry localization |
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