| 鸦胆子提取物对菜青虫幼虫蛋白酶活性和蛋白质含量的影响 |
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| 引用本文： | 曾涛,黎柳峰,韦德卫,陈海珊,刘演.鸦胆子提取物对菜青虫幼虫蛋白酶活性和蛋白质含量的影响[J].植物保护,2006,32(5):58-60. |
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| 作者姓名： | 曾涛 黎柳峰 韦德卫 陈海珊 刘演 |
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| 作者单位： | 1. 广西农业科学院植物保护研究所,南宁,530007
2. 中国科学院广西植物研究所,桂林,541006 |
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| 基金项目： | 国家自然科学基金;广西自然科学基金;广西农业科学院科技发展基金 |
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| 摘 要： | 鸦胆子氯仿和乙酸乙酯提取物对菜青虫幼虫蛋白酶的活性和蛋白质含量均有明显影响。测定结果表明,在10 mg/mL浓度处理下,氯仿和乙酸乙酯提取物处理8～20 h蛋白酶比活力均明显低于对照组。24 h时氯仿提取物的蛋白酶比活力(0.306 92μg/mg.min)为对照组(0.187 75μg/mg.min)的1.634 3倍;乙酸乙酯提取物的蛋白酶比活力(0.100 06μg/mg.min)为对照组(0.390 12μg/mg.min)的0.256 5倍。24 h时氯仿提取物的蛋白质含量(44.403 mg/mL)为对照组(81.457 mg/mL)的0.5 451倍;乙酸乙酯提取物的蛋白质含量(64.500 mg/mL)为对照组(205.495 mg/mL)的0.3 139倍。
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| 关 键 词： | 鸦胆子提取物 菜青虫 蛋白酶 蛋白质 |
| 收稿时间： | 2005-07-01 |
| 修稿时间： | 2005-07-012006-02-11 |
Effects of the extracts from Brucea javanica on protease activity and the protein contents of Pieris rapae |
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| Zeng Tao;Li Liufeng;Wei Dewei;Chen Haishan;Liu Yan.Effects of the extracts from Brucea javanica on protease activity and the protein contents of Pieris rapae[J].Plant Protection,2006,32(5):58-60. |
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| Authors: | Zeng Tao;Li Liufeng;Wei Dewei;Chen Haishan;Liu Yan |
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| Institution: | 1.Guangxi Academy of Agricultural Science;Nanning 530007;China;2.Institude of Botany;Guangxi Zhuangzu Autonomous Region and Academia Sinica;Guilin 541006;China |
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| Abstract: | The effects of the extracts from Brucea javanica using chloroform and ether acetate on the activity of protease and the protein contents of Pieris rapae were studied.The results showed that the protease activity was significantly lower than the control after treated with the extracts in chloroform and ether acetate at the concentration of 10 mg/mL,for 8-20 hours.At 24 hours after treated with the extracts in chloroform and ether acetate,the activity of protease was 163.43%,compared with 54.20% of the control,while the protein contents was 54.51%,compared with 31.39% of the control. |
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| Keywords: | Brucea javanica extracts Pieris rapae protease protein |
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