| 小麦条锈病不同抗性材料中分子标记YrY201-1分布研究 |
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| 引用本文： | 张胜利,李东方,胡海燕,赵俊杰,贺杰.小麦条锈病不同抗性材料中分子标记YrY201-1分布研究[J].广东农业科学,2011,38(3):115-116,120. |
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| 作者姓名： | 张胜利 李东方 胡海燕 赵俊杰 贺杰 |
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| 作者单位： | 河南科技学院生命学院/河南省高等学校作物分子育种重点学科开放实验室,河南,新乡,453003 |
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| 基金项目： | 河南省教育厅自然科学基础研究项目，河南科技学院高层次人才引进项目 |
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| 摘 要： | 以22个抗病品种、4个感病品种以及12个野生近缘材料的基因组DNA为模板进行PCR扩增,检测小麦抗条锈病基因分了标记YrY201-1的分布.结果表明:YrY201-1具有较高的扩增效率(96.2%,25/26);在4个感病材料中均能扩增出目标条带;在野生近缘材料中扩增效率也较高(91.7%,11/12),且在多数材料中扩增条带较多,除目标产物外还有许多非特异性扩增产物出现;在抗性基因YrY201的来源材料J11(粗山羊草)中该标记只扩增出1条特异性条带,但比预期目标条带稍大.
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| 关 键 词： | 条锈病 分子标记 |
Study on distribution of YrY201-1 molecular marker among different stripe rust resistance cultivars of wheat |
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| ZHANG Sheng-li,LI Dong-fang,HU Hai-yan,ZHAO Jun-jie,HE Jie.Study on distribution of YrY201-1 molecular marker among different stripe rust resistance cultivars of wheat[J].Guangdong Agricultural Sciences,2011,38(3):115-116,120. |
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| Authors: | ZHANG Sheng-li LI Dong-fang HU Hai-yan ZHAO Jun-jie HE Jie |
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| Institution: | ZHANG Sheng-li,LI Dong-fang,HU Hai-yan,ZHAO Jun-jie,HE Jie(Henan Institute of Science and Technology/Key Discipline Open Laboratory on Crop Molecular Breeding of Henan Institute of Higher Learning Xinxiang 453003,China) |
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| Abstract: | In this study,the DNA from 22 disease-resistant varieties,4 feeling cultivars and 12 wild relatives were used as templates and the distribution of molecular markers YrY201-1 of the stripe rust resistance gene YrY201 was speculated by PCR.The results showed that: the amplification of molecular marker YrY201-1 possessed high efficiency(96.2%,25/26).Target band could be amplified by this marker from all the 4 susceptible materials.The amplification efficiency of the 12 wild relatives was also very high(91.7%,1... |
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| Keywords: | YrY201-1 |
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