壳聚糖对狗牙根种子愈伤组织诱导和分化的影响

[目的]为优化狗牙根的组织培养体系。[方法]以普通狗牙根的成熟种子作外植体,研究了直接添加壳聚糖和用壳聚糖水溶液浸种对愈伤组织诱导和植株分化的影响。[结果]结果表明,N6培养基较适合用于诱导狗牙根种子的愈伤组织;壳聚糖的直接添加和浸种均能够显著提高愈伤组织的诱导率。其中,在N6培养基中,直接添加2 g/L壳聚糖时狗牙根种子出愈率最高,为88.00%。MS培养基较适合用于狗牙根种子愈伤组织的分化,添加1 g/L壳聚糖时愈伤组织分化率最高,为35.00%。

The Influence of Chitosan on the Induction and Differentiation of Bermuda grass

[Objective] The study aimed to optimize the tissue culture system for Bermuda grass.[Method] With mature seed of Bermuda grass as explant,the influence of directly addition of chitosan and soaking seeds in the water solution of chitosan on the callus induction and differentiation of Bermuda grass were studied.[Result] N6 medium was more fitted for the callus induction of Bermuda grass seeds,and directly addition of chitosan and soaking seeds in the water solution of chitosan all could obviously improve the callus induction rate.Callus rate of Common Bermuda grass seed in N6 medium with 2 g/L chitosan was highest(88.00%).MS medium was better for the callus differentiation of Common Bermuda grass,and the callus differentiation rate in the treatment with addition of 1 g/L chitosan was highest(35.00%).