| 超声照射对人胚肾细胞凋亡的影响 |
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| 引用本文： | 罗毅,王冉,徐晓红.超声照射对人胚肾细胞凋亡的影响[J].湛江医学院学报,2010,28(2):115-117. |
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| 作者姓名： | 罗毅 王冉 徐晓红 |
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| 作者单位： | 罗毅,王冉,LUO Yi,WANG Ran(广东医学院附属惠州中心人民医院功能科,广东惠州,516001);徐晓红,XU Xiao-hong(广东医学院附属医院超声科,广东湛江,524001) |
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| 基金项目： | 广东省卫生厅医学科研项目 |
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| 摘 要： | 目的了解诊断超声照射对体外培养的人胚肾（HEK-293）细胞凋亡的影响。方法 HEK-293细胞用腹部超声探头（3.5 MHz、MI 0.8）照射0、10、20、30 min,再作Hoechst 33258染色,观察细胞凋亡的变化。结果超声照射10 min后,细胞凋亡率与假照组比较差异无统计学意义（P〉0.05）;超声照射20 min后,细胞凋亡率明显高于假照组（P〈0.05）,其中以照射30 min较为显著（P〈0.01）。结论特定剂量的超声照射可诱导人胚肾细胞凋亡增加。
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| 关 键 词： | 超声 凋亡 形态学 |
Effect of ultrasound on apoptosis of human embryonic kidney cells |
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| LUO Yi,WANG Ran,XU Xiao-hong.Effect of ultrasound on apoptosis of human embryonic kidney cells[J].Journal of Guangdong Medical College,2010,28(2):115-117. |
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| Authors: | LUO Yi WANG Ran XU Xiao-hong |
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| Institution: | 1.Function Department,Huizhou Central People＇ s Hospital,Huizhou 516001,China) |
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| Abstract: | Objective To investigate the effect of diagnostic ultrasound exposure on the apoptosis of human embryonic kidney（HEK-293） cells.Methods HEK-293 cells were exposure to abdominal ultrasound probe for 0,10,20 and 30 min,and their apoptosis was detected by Hoechst 33258 staining.Results Compared with control group,apoptotic rate of HEK-293 cells increased significantly after 20-min exposure（P〈0.05）,especially after 30-min exposure（P〈0.01）.However,there was no statistical difference in apoptotic rate between 10-min exposure group and control group（P〉0.05）.Conclusion The dose of dia-gnostic ultrasound exposure can induce the apoptosis of HEK-293 cells. |
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| Keywords: | ultrasound apoptosis morphology |
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