两个洋葱雄性不育系细胞质类型鉴定及分析

 洋葱雄性不育系是选育洋葱杂交品种的关键材料，明确其细胞质类型以及起源，能为杂交组合的选配以及新型不育系的选育提供指导性意见。本文采用分子生物学手段，对从国内品种中选育出的两套洋葱细胞质雄性不育系进行了细胞质类型的鉴定，并用189个RAPD引物分析了不育系和相应保持系之间的多态性。结果表明，来自品种‘沙沟红皮’的不育系细胞质类型为T型，从‘朔州红皮’中选育出的雄性不育系为S型细胞质。对这两套近等位基因系的基因组随机扩增结果显示，与T雄性不育系相比，S型不育系与其保持系基因组之间有更多的差异。

Identification and Analysis of Cytoplasmic Male Sterility in Onion (Allium cepa L. ) Origin from Chinese Cultivars

Cytoplasmic male sterile line in Onion (Allium cepa L.) is crucial material for production of hybrid seeds. It is important to identify its cytoplasm and origination, for these could give breeder some guidance suggestions. In this paper, two sets of cytoplasmic male sterile lines, came from two different Chinese onion cultivars, were investigated by molecular method, and were discussed about their polymorphisms with corresponding maintainers. The result showed that the cytoplasms of male sterile lines origin from ‘Sha Gou Hong Pi’and ‘Shuo Zhou Zi Pi’are T and S, respectively. RAPD amplification showed that the S cytoplasm has more polymorphism than T cytoplasm compared to their maintainer lines.