肉牛IVF胚胎移植应用研究

;1999~2002年,应用肉牛IVF胚胎1000枚在当地7个移植点散养农户中移植受体母牛625头,总体受胎率为33.0%,已产活犊牛164头。其中诱导同期发情处理受体牛399头,发情217头,移植123头,受胎率25.2%;自然发情受体牛852头,移植502头,受胎率34.3%(P>0.05)。初步确定了应用国产氯前列烯醇(CLO)诱导同期发情的程序和剂量,对胚胎解冻方法和黄体功能与受胎率之间的关系进行了探讨,对不同品种、年龄和饲养管理方式的移植效果进行了比较,分析了受体牛移植后的再繁殖能力。

Research on application of beef cattle IVF embryo transplanting

From 1999 to 2002, 625 recipient cows which bred in scattered households on 7 local transplanting oints ,were transplanted with beef cattle IVF (in vitro fertilization ) frozen embryos about 1000 heads, The total eceptive rate of transplanting was 33.0 %, 164 calves were born from embryo transplanting during the past years. 99 recipient cows were treated with estrus synchronization, and 217 cases were successful, 123 cases were ransplanted, and the receptive rate was 25.2 %. 852 recipient cows were natural estrus and 502 cases were ransplanted, the receptive rate was 34.3 % (P>0.05). The optimum procedure and dosage were established for ynchronization estrus with Cloprostenol (CLO) made in china. Meanwhile, we studied the influence of embryo uality, unfrozen methods and corpus luteum function on the receptive rate of transplanting, compared with the eceptive rate of recipient cows with different breeds, ages and production process, and analyzed reproductivity of ecipient cows after embryo transplanting. The science criterion was provided to conduct beef IVF embryo ransplanting on industrial development at a large scale.