辅酶渣替代鱼粉对中华绒螯蟹生长及肠道菌群的影响

为探究饲料中辅酶渣替代鱼粉对中华绒螯蟹生长性能、健康情况和肠道菌群的影响，以中华绒螯蟹幼蟹(0.70±0.01g)为研究对象，分别以等蛋白的辅酶渣替代基础饲料中鱼粉的0%(对照组，含20%鱼粉)、20%、40%、60%、80%、100%(记为C、T20、T40、T60、T80、T100)的饲料投喂8周。结果显示，与C组相比，T40组生长性能无显著改变，T60组肌肉粗蛋白含量显著降低。T80组、T100组生长性能、体粗蛋白和粗脂肪含量显著降低；与C组相比，替代组血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(AKP)、甘油三酯(TG)水平未见显著差异，总蛋白水平(TP)在T100组显著降低。血清葡萄糖(Glu)在替代组中显著降低。T60、T80、T100组的血清总胆固醇(T-CHO)、高密度脂蛋白胆固醇(HDL-C)含量显著降低，T80、T100组的血清低密度脂蛋白胆固醇(LDL-C)含量显著降低；与C组相比，各替代组肝胰腺超氧化物歧化酶(SOD)活性无显著差异，谷胱甘肽过氧化物酶(GSH-Px)活性显著上升，T100组丙二醛(MDA)含量显著降低；C、T40、T80组肠道菌...

Growth, health and intestinal microbiota of Chinese mitten crab (Eriocheir sinensis) fed diets with coenzyme residue replacing fish meal

Coenzyme residue (CR) is a by-product of the production of coenzyme Q10. It is mainly obtained by fermentation products of Rhodobacter sphaeroides, glucose, and corn steep liquor. At present, it has been approved as a feed material for aquaculture of aquatic animals in China. To explore the effect of CR replacing fish meal in the compound feed of juvenile Chinese mitten crab (Eriocheir sinensis) on the growth performance, health and intestinal flora, juvenile crabs (0.70 ± 0.01 g) were fed in an 8-week feeding trial. On the basis of 20% fish meal level(control group), CR was used to replace 20%, 40%, 60%, 80%, 100% fish meal in the feed (recorded as C, T20, T40, T60, T80, T100). The results showed that, compared with C, the growth performance of T40 had no significant change, and the crude protein content of muscle in T60 was significantly reduced. The growth performance, body crude protein and crude fat content of T80 and T100 were significantly reduced; Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (AKP), triglyceride (TG) showed no significant difference among the groups. Total protein (TP) level was significantly decreased in T100. Serum glucose (Glu) was significantly decreased in the replacement groups. The serum levels of total cholesterol (T-CHO) and high-density lipoprotein cholesterol (HDL-C) in T60, T80 and T100 were significantly decreased, and the levels of low-density lipoprotein cholesterol (LDL-C) in T80 and T100 were significantly decreased. Compared with C, there was no significant difference in the activity of hepatopancreas superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity increased significantly. The content of malondialdehyde (MDA) decreased significantly in T100. Alpha diversity index showed no significant difference among three treatments (C, T40, T80). Principal coordinate analysis (PCoA) suggested that the microbial community structure in T80 group changed compared with the C group. In conclusion, CR can replace 40% fish meal in the feed of juvenile Eriocheir sinensis without affecting growth performance, antioxidant capacity and intestinal flora. When the replacement level reaches 80%, the growth performance and accumulation of nutrients of crab will be limited, and intestinal microbiota also changed. This experiment provides a theoretical reference for the application of CR in aquatic animal feed.