Common nucleotide sequence of structural gene encoding fibroblast growth factor 4 in eight cattle derived from three breeds |
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Authors: | Sho SATO Toshikiyo TAKAHASHI Hiroshi NISHINOMIYA Makiko KATOH Ryu ITOH Masaki YOKOO Mari YOKOO Momoe IHA Yuki MORI Kano KASUGA Ikuo KOJIMA Masayuki KOBAYASHI |
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Affiliation: | 1. Graduate School of Bioresource Sciences, Akita Prefectural University, Akita;2. Livestock Experiment Station, Akita Prefectural Agriculture, Forestry and Fisheries Research Center, Daisen, Japan |
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Abstract: | Fibroblast growth factor 4 (FGF4) is considered as a crucial gene for the proper development of bovine embryos. However, the complete nucleotide sequences of the structural genes encoding FGF4 in identified breeds are still unknown. In the present study, direct sequencing of PCR products derived from genomic DNA samples obtained from three Japanese Black, two Japanese Shorthorn and three Holstein cattle, revealed that the nucleotide sequences of the structural gene encoding FGF4 matched completely among these eight cattle. On the other hand, differences in the nucleotide sequences, leading to substitutions, insertions or deletions of amino acid residues were detected when compared with the already reported sequence from unidentified breeds. We cannot rule out a possibility that the structural gene elucidated in the present study is widely distributed in cattle. To the best of our knowledge, this is the first determination of the complete nucleotide sequence of the structural gene encoding bovine FGF4 in identified breeds. |
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Keywords: | amino acid sequence cattle FGF4 fibroblast growth factor 4 structural gene |
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