中国枣属植物亲缘关系的RAPD分析

 采用RAPD技术分析了原产我国的枣属14个种、枣的11个品种及1个外类群的DNA多态性。31个引物扩增出921条谱带, 919条(99.78% ) 表现出多态性。基于921个RAPD标记, 利用NTSYS软件计算Dice遗传相似系数, 并建立UPGMA聚类图, 结果表明: 在0.26阈值处将供试材料分为6类, 不支持前人根据单一性状对枣属属下组、系类群的划分; 枣和酸枣、蜀枣和山枣宜分别作同一个种处理, 同时提出枣种下宜划分为两个亚种, 并给出了新等级的学名; 支持将枣种下传统划分的变种并入原变种。

Studies on the Phylogenetic Relationships of Chinese Ziziphus by RAPD Tech-nique

The genomic DNA polymorphisms of 14 species of Chinese Ziziphus, 11 varieties of Z. jujuba Mill. and one outgroup were analyzed by using random amp lified polymorphic DNA (RAPD). A total of 921 RAPD bandswere amplified by 31 random p rimers, amongwhich 919 (99.78% ) were polymorphic. The data of 921 RAPD bands were used to generate Diceps similarity coefficients and to construct a dendrogram by means of UPGMA in the NTSYS-pc program. It is concluded as follows: All samples were classified into six kinds at genetic similarity 0126. The results canpt support the classification of Sect. and Ser. on Z iziphus that based on single character. Z. jujubaMill. and Z. acidojujuba C. Y. Cheng et M. J. L iu. should be treatedas one species. In the same way, Z. xiangchengensis Y. L. Chen et P. K. Chou and Z. montana W. W.Smith should be treated as one species. Infraspecific classification of Z. jujubaMill. should be classified into two subspecies, which were given new Latin names. It is agreed that the varieties of Z. jujuba should be merged into Z. jujuba.