| 应用PCR技术检测柞蚕微孢子虫 |
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| 引用本文： | 邓真华,姜义仁,杨瑞生,张涛,秦利,姜德富. 应用PCR技术检测柞蚕微孢子虫[J]. 蚕业科学, 2010, 36(2): 359-362. DOI: 10.3969/j.issn.0257-4799.2010.02.028 |
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| 作者姓名： | 邓真华 姜义仁 杨瑞生 张涛 秦利 姜德富 |
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| 作者单位： | 沈阳农业大学柞蚕研究所,沈阳,110866;辽宁省蚕业科学研究所,辽宁凤城,118100 |
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| 基金项目： | 现代农业产业技术体系建设专项(蚕桑)项目 |
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| 摘 要： | 采用斑迹抽提法提取柞蚕微孢子虫(Nosema pernyi)基因组DNA,基因组DNA的琼脂糖凝胶电泳图谱中有大小约15kb的清晰、完整条带。选用已报道的微粒子属16S rRNA基因的保守序列设计P1/P2和N1/N22对引物,对柞蚕微孢子虫基因组DNA进行PCR扩增,结果2对引物分别扩增出1条大小不同的特异谱条带,其中用引物N1/N2扩增可检测出0.47ng的DNA模板。应用同样的PCR引物、体系和反应条件,可有效扩增出受感染柞蚕幼虫、成虫中的微孢子虫基因组DNA条带。该项检测技术有望应用于柞蚕微粒子病的早期诊断。
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| 关 键 词： | 柞蚕 微粒子病 柞蚕微孢子虫 PCR诊断 |
Application of PCR Technology in the Inspection of Nosema pernyi |
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| DENG Zhen-Hua,JIANG Yi-Ren,YANG Rui-Sheng,ZHANG Tao,QIN Li,JIANG De-Fu. Application of PCR Technology in the Inspection of Nosema pernyi[J]. Acta Sericologica Sinica, 2010, 36(2): 359-362. DOI: 10.3969/j.issn.0257-4799.2010.02.028 |
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| Authors: | DENG Zhen-Hua JIANG Yi-Ren YANG Rui-Sheng ZHANG Tao QIN Li JIANG De-Fu |
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| Affiliation: | DENG Zhen-Hua1 JIANG Yi-Ren1 YANG Rui-Sheng1 ZHANG Tao1 QIN Li1 JIANG De-Fu2(1Institute of Tussah Silkworm,Shenyang Agricultural University,Shenyang 110866,China,2The Sericultural Research Institute of Liaoning Province,Fengcheng Liaoning 118100,China) |
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| Abstract: | The genomic DNA of Nosema pernyi was extracted by means of disease-spot method.The genomic DNA band with a length of around 15 kb was clear and intact in the agarose gel electrophoretogram.PCR amplifications using two pairs of primers(P1/P2 and N1/N2)designed in accordance with conserved regions of the reported Nosema 16S rRNA genes to amplify the genomic DNA showed that both primer pairs yielded one specific DNA band of different size respectively from the genomic DNA of Nosema pernyi,among which amplifica... |
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| Keywords: | Antheraea pernyi Pebrine disease Nosema pernyi PCR diagnosis |
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