| 板栗叶片的DNA提取 |
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| 引用本文： | 翁尧富,陈源,郑康乐,陆军,赵勇春. 板栗叶片的DNA提取[J]. 浙江林业科技, 1999, 0(3) |
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| 作者姓名： | 翁尧富 陈源 郑康乐 陆军 赵勇春 |
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| 作者单位： | 浙江省林业厅种苗站!杭州，310004(翁尧富，陈源，赵勇春)，中国水稻研究所(郑康乐，陆军) |
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| 基金项目： | 浙江省科委“九五”重点攻关课题 |
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| 摘 要： | 板栗叶片是一种多糖和次级混合量均较高的植物材料，DNA提取的关键步骤是使用十六烷基三甲基溴化铵（CTAB）和聚乙烯吡咯啉酮（PVP）。经本文所描述的方法得到的DNA，完全能满足随机扩增多态性DNA（RAPD）分析。
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| 关 键 词： | 板栗叶片 DNA提取 |
Drawing DNA from Blades of Castanea mollissima |
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| Weng Yaofu,Chen Yuan,Zheng Kangle,Lu Jun,Zhao Yongchun. Drawing DNA from Blades of Castanea mollissima[J]. Journal of Zhejiang Forestry Science and Technology, 1999, 0(3) |
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| Authors: | Weng Yaofu Chen Yuan Zheng Kangle Lu Jun Zhao Yongchun |
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| Affiliation: | Seeding Station of Thejiang Forertry Department Hangzhou 310004;Rice Institute of China |
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| Abstract: | Blades of Castanea mollissima contain rich polysaccharide and secondary mixture key proccedure of drawing DNA was utilization of CTAB and pvp. The DNA drown by the method in this paper could completely suitable for RAPD analysis. |
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| Keywords: | blades of Castanea mollissima DNA drawing |
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