| Analysis of Two-Dimensional Gel Electrophoresis Images of Protein from Posterior Silk Gland of Silkworm （Bombyx mort） on Day 1 and Day 4 in the 5th Instar Stage |
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| 引用本文： | WU Wei-cheng GAOQi-kang CHEN Jin-e YE Jian QIAN Yang-wen LI Jian-ying LU Hua-yun MENG Zhi-qi NI Chun-xiao ZHONG Bo-xiong. Analysis of Two-Dimensional Gel Electrophoresis Images of Protein from Posterior Silk Gland of Silkworm （Bombyx mort） on Day 1 and Day 4 in the 5th Instar Stage[J]. 中国农业科学(英文版), 2007, 6(2): 249-254. DOI: 10.1016/S1671-2927(07)60042-X |
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| 作者姓名： | WU Wei-cheng GAOQi-kang CHEN Jin-e YE Jian QIAN Yang-wen LI Jian-ying LU Hua-yun MENG Zhi-qi NI Chun-xiao ZHONG Bo-xiong |
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| 作者单位： | [1]Laboratory of Molecular Biology of Silkworm and Honeybee~College of Animal Science, Zhejiang University, Hangzhou 310029, P.R. China [2]Institute of Applied Entomology, Zhejiang University, Hangzhou 310029, P.R.China [3]College of Agriculture ＆ Biotechnology, Zhejiang University, Hangzhou 310029, P.R.China [4]Department of Speciality Good Seed Extension, Hangzhou 310002, P.R. China [5]Laboratory of Molecular Biology of Insect, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P.R.China |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | The posterior silk gland （PSG） of silkworm is an important organ where fibroin is synthesized and secreted exclusively. Because fibroin constitutes 75-80% of the silk filament, the mechanism governing fibroin secretion, quality and yield of cocoon can be elucidated by the study on the PSG. Using two-dimensional gel electrophoresis （2-DE） and image analysis system, the changes in the protein composition in the PSG cell were investigated on the day 1 （D1） and day 4 （D4） in the 5th instar stage from five different strains of silkworm （Bombyx mori）. While differences at protein level between days and strains were far less than those observed at the gene level using EST analysis. The change trends in protein composition from D1 to D4 were diverse among the different strains. The results suggest that the secretion of fibroin is regulated by multiple proteins. The site of regulation and the proteins responsible for the regulation vary with the strain, which leads to differences between strains in the capacity of fibroin secretion in the PSG cell.
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| 关 键 词： | 蚕 5龄期 丝腺 蛋白 二维凝胶电泳 图象分析 |
Analysis of Two-Dimensional Gel Electrophoresis Images of Protein from Posterior Silk Gland of Silkworm (Bombyx mori) on Day 1 and Day 4 in the 5th Instar Stage |
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| WU Wei-cheng,GAO Qi-kang,CHEN Jin-e,YE Jian,QIAN Yang-wen,LI Jian-ying,LU Hua-yun,MENG Zhi-qi,NI Chun-xiao,ZHONG Bo-xiong. Analysis of Two-Dimensional Gel Electrophoresis Images of Protein from Posterior Silk Gland of Silkworm (Bombyx mori) on Day 1 and Day 4 in the 5th Instar Stage[J]. 《Agricultural Sciences in China》, 2007, 6(2): 249-254. DOI: 10.1016/S1671-2927(07)60042-X |
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| Authors: | WU Wei-cheng GAO Qi-kang CHEN Jin-e YE Jian QIAN Yang-wen LI Jian-ying LU Hua-yun MENG Zhi-qi NI Chun-xiao ZHONG Bo-xiong |
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| Affiliation: | 1. Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA;2. Molecular and Cellular Biology Program, Oregon State University, Corvallis, OR 97331, USA;3. Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR 97331, USA;4. Environmental Health Sciences Center, Oregon State University, Corvallis, OR 97331, USA;5. School of Biological and Population Health Sciences, Oregon State University, Corvallis, OR 97331, USA;1. Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL 32611, USA;2. Department of Civil and Environmental Engineering and Nicholas School of the Environment, Duke University, Durham, NC 27708, USA;3. Department of Environmental and Global Health and Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL 32611, USA |
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| Abstract: | The posterior silk gland (PSG) of silkworm is an important organ where fibroin is synthesized and secreted exclusively.Because fibroin constitutes 75-80% of the silk filament, the mechanism governing fibroin secretion, quality and yield of cocoon can be elucidated by the study on the PSG. Using two-dimensional gel electrophoresis (2-DE) and image analysis system, the changes in the protein composition in the PSG cell were investigated on the day 1 (D1) and day 4 (D4) in the 5th instar stage from five different strains of silkworm (Bombyx mori). While differences at protein level between days and strains were far less than those observed at the gene level using EST analysis. The change trends in protein composition from D1 to D4 were diverse among the different strains. The results suggest that the secretion of fibroin is regulated by multiple proteins. The site of regulation and the proteins responsible for the regulation vary with the strain, which leads to differences between strains in the capacity of fibroin secretion in the PSG cell. |
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| Keywords: | silkworm (Bombyx mori) silk gland protein 2-DE image analysis |
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