| 一种基于模板DNA的PCR扩增难易预测方法 |
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| 引用本文： | 范秀静,李楠,李春. 一种基于模板DNA的PCR扩增难易预测方法[J]. 浙江农业学报, 2012, 24(4): 718-720 |
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| 作者姓名： | 范秀静 李楠 李春 |
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| 作者单位： | 渤海大学数理学院,辽宁锦州,121000 |
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| 摘 要： | 借助赋予四种碱基A,G,C,T四个三维空间中的点(0,0,1),(0,1,0),(-1,-1,0),(1,0,0),将一条DNA序列转化为一个二十四维向量。在此基础上利用Fisher线性判别法对人类基因组中189条基因片段进行PCR扩增难易预测,识别率达到了90%。
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| 关 键 词： | 模板DNA 24维向量 聚合酶链式反应(PCR) |
A predictive method for polymerase chain reactions based on the template DNA |
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| FAN Xiu-jing , LI Nan , LI Chun. A predictive method for polymerase chain reactions based on the template DNA[J]. Acta Agriculturae Zhejiangensis, 2012, 24(4): 718-720 |
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| Authors: | FAN Xiu-jing LI Nan LI Chun |
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| Affiliation: | (College of Mathematics and Physics,Bohai University,Jinzhou 121000,China) |
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| Abstract: | By means of assigning(0,0,1),(0,1,0),(-1,-1,0),(1,0,0) to the four bases A,G,C and T,a DNA sequence is transformed into a 24-dimensional vector.Based on this and the Fisher linear discriminant algorithm,we proposed a computational method to predict success for PCR.Our report is based on 189 amplicons randomly selected from human exons,and our method achieves an accuracy of 90%. |
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| Keywords: | template DNA 24-dimensional vector polymerase chain reactions(PCR) |
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