小白菜高效子叶离体植株再生体系的建立

以丰顺小白菜带柄子叶为外植体,研究了苗龄、不同激素配比、A gNO3浓度和抗生素、共培养时间对不定芽再生的影响。结果显示,子叶不定芽再生的最佳苗龄为5~9d;经过2d的预培养,在M S 1.0m g/L TDZ 0.1m g/L NAA 7.5m g/L A gNO3 0.5m g/L ABA培养基中,带柄子叶不定芽再生率最高,为82.53%;在含有2.0m g/LIBA的1/2M S0培养基中诱导生根,不定根形成率为100%。抗生素敏感性试验表明,可选择浓度为20m g/L Hyg(潮霉素)作为最适合选择压,抑制农杆菌生长的抗生素选用500m g/L Cb(羧卞青霉素)。不同浓度农杆菌菌液对子叶再生不定芽的影响不大,但共培养时间的长短却明显地影响不定芽的分化。

Development of high frequency plantlet regeneration system for in vitro culture of cotyledons of Chinese cabbage

The cotyledons with petiole from Fengshun Chinese cabbage were used as explants,and the effects of seedling age and different homone combinations,AgNO_3 concentration and antibiotics,co-culture days with Agrobactium tumefaciens on the regeneration of adventitious bud of cotyledon were studied.The results indicated that the best seedling age to induce adventitious bud regeneration was 5-9 days,and the regeneration rate of cotyledon was the highest with 82.53% in medium MS 1.0mg/L TDZ 0.1mg/L NAA 7.5mg/L AgNO_3 0.5mg/L ABA after sub-cultured for two days;the formation rate of adventitious root was 100% in 1/2MS_0medium containing 2.0mg/L IBA for root induction.The results of antibiotics sensitivity experiments indicated that 20mg/L Hyg was the most appropriate for selective pressure and 500mg/L Cb was used to restrain the growth of Agrobactium tumefaciens.In addition,the differentiation of adventitious buds was evidently affected by co-culture days,but had no close relation with the concentration of Agrobactium tumefaciens.