大豆泛变应原profilin基因的克隆、序列分析和表位预测

通过生物信息学方法对多种植物性食物和花粉泛变应原proflin基因进行比对,利用其中的高度保守区域设计并合成简并引物,通过RT-PCR克隆得到一个新的大豆profilin基因(396bp,pⅠ为5.21,MW为14,1kD),序列同源性分析发现该基因和已知的多种植物性食物、花粉变应原profilin有较高的同源性(＞75%),因此认为其系大豆蛋白泛变应原profilin,命名为Gly m 3.02,EMBL/GeneBank数据库登录号为DQ784852,这为进一步重组表达和抗原表位分析等奠定了实验基础.

CLONING, SEQUENCE ANALYSIS AND ANTIGEN EPITOPE PREDICTION OF PANALLERGEN PROFILIN IN SOYBEAN

Bioinformatic method was used for the comparative analysis of numerous homologous plant food panallergen and pollen panallergen sequences.Conservative domains among the sequences were determined for degenerate primer designing.The defined RT-PCR conditions were adopted to clone the full-length gene of profilin from soybean and a novel full-length cDNA clone(396bp,pI 5.21,MW 14,1kD) was obtained.Sequence analysis showed that this gene shared high identities(>75%) with panallergen profilin from some plant food and pollen.The deduced protein was therefore regarded as panallergen and named as Gly m 3.02(EMBL/GenBank database entry No.DQ784852).This novel panallergen profilin gene from soybean will be a base for the expression of allergen proteins and analysis of epitope applied in further studies and clinical treatment.