酶的选择性纯化及酶解制备木低聚糖的研究

采用沉淀剂的Ｈ处理木聚糖酶及纤维素酶以除去大部分β－木糖苷酶，以及酶解制备木低聚糖的研究结果。研究表明，当酶液中沉淀剂Ｈ的孢和度为５０％时，处理效果最佳。

STUDY ON XYLO-OLIGOSACCHARIDES PRODUCTION FROM XYLAN BY HYDROLYSIS OF SELECTIVELY PURIFIED ENZYMES

Xylooligosaccharides production from xylan by hydrolysis of selectively purified xylanase or cellulase synthesized by Trichoderma reesei Rut C30 was reported. Addition of precipitant H was effective to remove xylosidase from xylanase or cellulase system. Through comparison with one type of xylanase and two types of cellulase, it was found that precipitant H treatment was more suitable for xylanase. The results showed that the yield of xylose, in the products of hydrolysis by xylanase treatment was kept at a rather low level(<1 %) and the oligosaccharide yield was stable. The saturation degree of precipitant H at 30 %60 % were suitable to remove xylosidase, while 50 % was the optimum. It was concluded that the effective conversion of xylan was enhanced and the ratio of oligosaccharides to xylose was markedly increased with this method, and this technique would provide a new way for oligosaccharide production.