| 工布乌头中三种生物碱HPLC法测定优化 |
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| 引用本文： | 王若丁,王 超,孟凡娟,等.工布乌头中三种生物碱HPLC法测定优化[J].黑龙江农业科学,2014(1):106-109. |
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| 作者姓名： | 王若丁 王 超 孟凡娟 等 |
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| 作者单位： | [1]东北林业大学生命科学学院,黑龙江哈尔滨150040 [2]西藏大学农牧学院,西藏林芝860000 [3]西藏自治区质量技术监督局,西藏拉萨850000 |
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| 基金项目： | 国家“卜二五”科技支撑计划资助项目（2011BAI13806） |
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| 摘 要： | 为优化工布乌头中生物碱的测定方法,采用HPLC法测定工布乌头中新乌头碱、塔拉萨敏和乌头碱的含量。结果表明:采用10%氨水1mL、乙醚20mL,浸泡24h后超声处理30min可有效地对工布乌头中生物碱进行定量分析。
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| 关 键 词： | 工布乌头 高效液相色谱 新乌头碱 塔拉萨敏 乌头碱 |
The Optimization of HPLC Determination for Three Kinds of Alkaloids in Aconitum kongboense L. |
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| WANG Ruo-ding,WANG Chao,MENG Fan-juan,PENG Guang-hua,LAN Xiao-zhong.The Optimization of HPLC Determination for Three Kinds of Alkaloids in Aconitum kongboense L.[J].Heilongjiang Agricultural Science,2014(1):106-109. |
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| Authors: | WANG Ruo-ding WANG Chao MENG Fan-juan PENG Guang-hua LAN Xiao-zhong |
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| Institution: | 1. College of Life Science, Northeast Forestry University, Harbin, Heilongjiang 150040 ; 2. Agriculture and Animal Husbandry College, Tibet university, Linzhi, Tibet 860000 ; 3. Tebit Bureau of Quality and Technical Supervision,Lhasa,Tibet 850000) |
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| Abstract: | In order to optimize the extracting method of alkaloids in Aconitum kongboense L .,the content of mesaconitine ,talatisamine and aconitine were determined by HPLC .The results showed that 10% ammonia 1 mL ,ether 20 mL ,soak for 24 h ,30 min after ultrasonic treatment could be used to the quantitative analysis of the alkaloids of A conitum kongboense L .. |
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| Keywords: | Aconitum kongboense L high performance liquid chromatography （HPLC） mesaconitine Talati-samine aconitine |
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