异亮氨酸对嗜酸氧化亚铁硫杆菌胞外多聚物的影响

为提高生物冶金效率,研究了添加异亮氨酸对嗜酸氧化亚铁硫杆菌胞外多聚物组分、嗜酸氧化亚铁硫杆菌生长和吸附能力的影响。结果表明:与对照相比,添加异亮氨酸的嗜酸氧化亚铁硫杆菌生长周期明显缩短,Fe2+的耗尽时间缩短,培养后期Fe2+剩余量明显降低。最高菌密度提高17%,环境pH降低20.45%,胞外多聚物中蛋白质组分提高16.84%,多糖组分提高5.05%。培养时间为72h时,添加异亮氨酸的嗜酸氧化亚铁硫杆菌菌液EPS吸附量为54.520 8μg·mL-1,比对照提高了44.05%。整个生长周期中,异亮氨酸对菌液中游离EPS含量影响不大。

Effect of Isoleucine on Extracellular Polymeric Substance of Acidithiobacillus f errooxidans

In order to improve the efficiency of biological metallurgy ,the effect of isoleucine added to the liquid medium on extracellular polymeric component of Acidithiobacillus ferrooxidans ,growth and adsorption capac-ity were studied .The results showed that compared with CK ,growth cycle of Acidithiobacillus ferrooxidans with isoleucine and consumption time of Fe2＋ were shortened ,the highest cell density increased by 17% ,pH of medium decreased by 20 .45% ,protein content of extracellular polymeric increased by 16 .84% ,polysaccharide content increased by 5 .05% .The adsorption amount of EPS added isoleucine was 54 .520 8 μg·mL^-1 for 72 h , increased by 44 .05% than CK .During the whole growth cycle ,isoleucine had little effect on dissociation of EPS .