| 4种信号分子处理后马铃薯Star基因表达的RT-PCR分析 |
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| 引用本文： | 吴田,谢从华. 4种信号分子处理后马铃薯Star基因表达的RT-PCR分析[J]. 华中农业大学学报, 2010, 29(3): 268-271 |
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| 作者姓名： | 吴田 谢从华 |
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| 作者单位： | 1. 西南林学院园林学院,昆明,650224
2. 华中农业大学园艺林学学院,武汉,430070 |
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| 基金项目： | 国家"863"项目，西南林学院园林植物与观赏园艺云南省重点学科基金 |
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| 摘 要： | 以马铃薯叶片为试验材料,其叶片分别经外源SA、MJ、ETH、ABA处理后,在不同时间点取样,提取总RNA,对Star基因的表达进行半定量RT-PCR分析.结果表明,Star基因能够被SA、MJ、ABA迅速诱导表达,而被ETH诱导表达的时间比较晚.可见,Star基因被4种信号分子诱导表达之间存在广泛的重叠,反映了在马铃薯抵御晚疫病菌侵染过程中Star基因参与了一个非常复杂的信号传递过程.
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| 关 键 词： | 马铃薯 富含锚蛋白重复结构域 晚疫病 基因表达 |
| 收稿时间： | 2009-11-25 |
| 修稿时间： | 2009-03-22 |
RT-PCR Analysis of Expression of Star Gene in Potatoes Induced by Four Signal Molecules |
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| WU Tian and XIE Cong-hua. RT-PCR Analysis of Expression of Star Gene in Potatoes Induced by Four Signal Molecules[J]. Journal of Huazhong Agricultural University, 2010, 29(3): 268-271 |
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| Authors: | WU Tian and XIE Cong-hua |
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| Affiliation: | College of Horticulture and Gardening,Southwest Forestry University,Kunming 650224,China; and College of Horticulture and Forestry,Huazhong Agricultural University,Wuhan 430070,China |
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| Abstract: | Potato plants were treated with signal molecules SA,MJ,ETH and ABA.The leaves were sampled at different time points and the total RNA was extracted for semi-RT-PCR analysis.The relative expressions of Star gene under the four signal molecules were analyzed.The results showed that Star gene could be rapidly induced by SA,MJ and ABA but slowly by ETH.The expressions of Star gene induced by the four signal molecules were widely overlapped,suggesting that this gene could take part in a very important process of signal transmission in response to Phytophthora infestans infest. |
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| Keywords: | potato ankyrin-rich repeat gene late blight gene expression |
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