水稻籼粳特异性RFLP标记及广亲和品种亲缘关系分析

 应用160个RFLP标记分析了21个水稻广亲和品种6个籼粳测验种，发现其中68个标记可以区分籼粳测验种。21个广亲和品种根据与籼粳测验和共有片段比率的大小 可以分为籼，粳和籼粳中间型三类。68个标记在15个籼粳品种中进一步筛选，得到24个籼粳特异性RFLP标记。它们在亚种内杂交带型相同而亚种产不一样。其中RG358、G318为籼稻专一性探针，在粳稻中表现为零等位。以此24个探针为基础构建了广亲和品品种亲缘关系的树状图。讨论了籼粳特异性RFLP标记在水稻遗传育种实践中的应用。

Screening of Subspecies Differentiating RFLP Markers and Phylogenetic Analysis of Wide Compatibility Varieties in Oryza sativa

One hundred and sixty DNA clones were used to survey RFLPs among 21 wide compatibility varieties(WCVs) and 6 indica and japonica testers of Oryza sativa L. Sixty-eight clones distinguishing indica from japonica testers were detected. Based on ratios of shared fragments between WCVs and testers, 21 WCVs were divided into three groups: indica,japonica and intermediate. By further-screening among 15 indica and japonica varieties, 24 among 68 clones were found to be indica-japonica differentiating Probes. They show same hybridization patterns within subspecies, but different patterns between the subspecies. RG358, G318 were confirmed to be indica specific clones, which showed null alleies in japonica varieties. A phylogenetic tree of 21 WCVs was constructed based on these 24 indica-Japonica differentiating probes and the significance of these probes in rice genetics and breeding pyactice was discussed.